文献类型：期刊文献

中文题名：全萼秦艽的生药学研究

英文题名：Pharmacognostic Studies on Gentiana lhassica

作者：汪荣斌[1,2];张西玲[2];刘丽莎[2]

第一作者：汪荣斌

机构：[1]安徽中医药高等专科学校,安徽芜湖241000;[2]甘肃中医药大学,兰州730000

第一机构：安徽中医药高等专科学校,安徽芜湖241000

年份：2016

卷号：22

期号：9

起止页码：21

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家中医药管理局科技专项(04-05ZL23)

语种：中文

中文关键词：秦艽;全萼秦艽;微形态;外部形态;内部构造;獐牙菜苦苷;龙胆苦苷

外文关键词：Gentiana macrophylla; G.lhassica; micromorphology; external morphology; internal structure; swertiamarin; gentiopicroside

摘要：目的:对全萼秦艽和秦艽进行生药学的比较研究,为全萼秦艽的进一步开发和利用提供参考。方法:利用生药学鉴定方法,从微形态、形态及理化等方面对全萼秦艽和秦艽进行系统分析和评价。采用HPLC测定獐牙菜苦苷和龙胆苦苷含量,流动相甲醇-水梯度洗脱,检测波长254 nm。结果:全萼秦艽的内、外周皮的木栓细胞只有1层。全萼秦艽及秦艽中龙胆苦苷质量分数分别为6.24%和8.51%,獐牙菜苦苷依次为0.26%和0.36%。结论:全萼秦艽在生药学特征方面与秦艽存在部分差异,为全萼秦艽资源的开发利用提供了实验依据。
Objective： Gentiana lhassica was compared with G. macrophylla in their pharmacognosy.Method： Quality of G. lhassica from some aspects of micromorphology was evaluated, on morphological and physicochemical identification by pharmacognostic determination. HPLC was employed to determine contents of swertiamarin and gentiopicroside with mobile phase of methanol-water for gradient elution and detection wavelength at254 nm. Result： Cork cells of G. lhassica had only one layer in the inner peripheral skin and peripheral skin. The content of gentiopicroside was 6. 24%,higher than standard of the 2015 edition of Chinese Pharmacopoeia. The content of swertiamarin was 0. 26%. Conclusion： There are some differences between G. lhassica and G.macrophylla in pharmacognostic character. This study provides important reference for exploitation and utilization of G. lhassica.