文献类型：期刊文献

英文题名：Mechanisms of miR-195-5p and FOXK1 in rat xenograft models of non-small cell lung cancer

作者：Niu, Jiguo[1];Wang, Yiwen[2];Hu, Yonghua[3];Li, Caili[4];Fang, Yue[5]

第一作者：Niu, Jiguo

通信作者：Wang, YW[1]

机构：[1]Gansu Prov Canc Hosp, Dept Nucl Med, Lanzhou 730050, Gansu, Peoples R China;[2]Gansu Prov Canc Hosp, Dept Clin Lab, 2 Xiaoxihu East St, Lanzhou 730050, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[4]Northwest Minzu Univ, Sch Med, Lanzhou 730030, Gansu, Peoples R China;[5]Gansu Prov Hosp TCM, Lanzhou 730050, Gansu, Peoples R China

第一机构：Gansu Prov Canc Hosp, Dept Nucl Med, Lanzhou 730050, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Canc Hosp, Dept Clin Lab, 2 Xiaoxihu East St, Lanzhou 730050, Gansu, Peoples R China.

年份：2021

卷号：13

期号：4

起止页码：2528

外文期刊名：AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH

收录：;Scopus(收录号：2-s2.0-85105279177);WOS:【SCI-EXPANDED(收录号:WOS:000647700000010)】；

基金：The Natural Science Foundation of Gansu Province, project number 17JR5RA169, Technical Research and Development Special Project of Gansu Province No. 1105TCYA019 and Scientific research project of colleges and universities in Gansu Province Project number 2016A-045.

语种：英文

外文关键词：Non-small cell lung cancer; miR-195-5p; FOXK1; mechanism

摘要：Objective: To investigate the roles and mechanisms of miR-195-5p and forkhead box K1 (FOXK1) in rat xenograft models of non-small cell lung cancer (NSCLC). Methods: Rat xenograft models of NSCLC were established. Evaluations of morphology of NSCLC cells and levels of Ki67 and P53 were detected by hematoxylin-eosin (HE) staining and immunohistochemistry (IHC), respectively. The miR-195-5p level in NSCLC was measured by quantitative real-time RT-PCR (qRT-PCR), and FOXK1, Bax, Caspase-3 and Bal-2 levels were quantified by Western blot. And the regulatory relation between miR-195-5p and FOXK1 was determined by dual-luciferase reporter (DLR) assay. Results: HE staining and IHC demonstrated successful establishment of NSCLC models in which miR-195-5p was downregulated and FOXK1 was upregulated. Pearson correlation showed that miR-195-5p and FOXK1 were inversely associated (r=0.551, P=0.012). DLR assay confirmed the targeted regulatory relation between miR-195-5p and FOXK1, and upregulation of miR-195-5p accelerated apoptosis of tumor cells. Conclusion: miR-195-5p is inversely associated with FOXK1 in NSCLC in rats. Upregulation of miR-195-5p suppresses FOXK1 and accelerates apoptosis of tumor cells, which may serve as an therapeutic target for the treatment of NSCLC.