文献类型：期刊文献

英文题名：Isolation, characterization, and LC MS/MS determination of anti-obesity components from pine needles of Cedrus deodara (Roxb.) G. Don

作者：Wang, Xin[1];Li, Bin[1];Liu, Dongyan[2];Guo, Yuer[3];Zhang, Jiaxu[1];Li, Wanyu[1];Peng, Tengteng[4];Ma, Quhuan[2];Shi, Xiaofeng[1,2]

第一作者：王馨;王昕;王欣;王鑫

通信作者：Shi, XF[1];Ma, QH[2];Shi, XF[2]

机构：[1]Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]Gansu Acad Med Sci, Inst Pharmaceut Res, Lanzhou, Peoples R China;[3]Lanzhou Univ Technol, Coll Biomed, Lanzhou, Peoples R China;[4]Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]corresponding author), Gansu Acad Med Sci, Inst Pharmaceut Res, Lanzhou, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2024

卷号：11

外文期刊名：FRONTIERS IN NUTRITION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001291616400001)】；

基金：The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The authors acknowledged financial support from the Gansu Province Science and Technology Program Grant Project (No. 23CXGA0038) and the Natural Science Foundation of Gansu Province (No. 23JRRA1802). DAS:The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding authors.

语种：英文

外文关键词：Cedrus deodara (Roxb.) G. Don; needle leaves; anti-obesity ingredients; phenolic acids; UPLC-QQQ-MS/MS

摘要：Objective: This study aimed to isolate and analyze the components in cedar pine needles (needle leaves of Cedrus deodara (Roxb.) G. Don) that exhibit anti-obesity effects, as determined through animal experiments. Methods: The extract of cedar pine needles was separated into four fractions of different polarities using a macroporous resin column. The fraction that retained anti-obesity activity was evaluated based on the results of animal experiments. Monomeric compounds were structurally characterized and isolated from the active fraction using a preparative liquid chromatography system. Combined with subsequent glucose gel chromatographic separation. The content of the separated components was determined using ultrahigh performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS). Results: The water-washed fraction retained anti-obesity activity of the cedar pine needles more effectively. A total of 16 compounds were separated from this fraction, and the contents of 14 of these compounds were determined to be present in cedar pine needles. Conclusion: Nine components, namely p-hydroxy benzyl alcohol, chlorogenic acid, vanillic acid, syringic acid, P-coumaric acid, sinapic acid, benzoic acid, phenylacetic acid, salicylic acid, were characterized and determined for the first time in cedar pine needles. The components with anti-obesity activity in the pine needles of Cedrus are mainly derived from phenolic acids.