详细信息

HPLC测定不同产地当归中阿魏酸的含量     被引量:25

Determination of Ferulic Acid Content in DangGui from Different Habitats by HPLC

文献类型:期刊文献

中文题名:HPLC测定不同产地当归中阿魏酸的含量

英文题名:Determination of Ferulic Acid Content in DangGui from Different Habitats by HPLC

作者:李成义[1];王延惠[1];魏学明[1];李硕[1];王明伟[1]

第一作者:李成义

机构:[1]甘肃中医学院,甘肃兰州730000

第一机构:甘肃中医药大学

年份:2012

卷号:25

期号:1

起止页码:34

中文期刊名:西部中医药

外文期刊名:Western Journal of Traditional Chinese Medicine

收录:CSTPCD

基金:2009年农牧厅甘肃省中药材产业攻关项目(编号BH2010-093)

语种:中文

中文关键词:当归;阿魏酸;HPLC

外文关键词:DangGui; ferulic acid; HPLC

摘要:目的:采用高效液相色谱法测定甘肃不同产地当归中阿魏酸含量,并对不同产地药材质量做客观比较。方法:用高效液相色谱法,使用ZORBAX Edipse XDB-C18柱(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈∶0.085%水溶液(17∶83)(V/V);流速为1.0 mL/min;进样量为10μL;柱温为:35℃;检测波长为316 nm。结果:3个不同产地当归中阿魏酸含量有明显差异,岷县>漳县>哈达铺,其中岷县当归阿魏酸含量为0.1206%。3个不同产地当归阿魏酸含量均高于药典标准。结论:甘肃不同产地当归阿魏酸含量均高于药典标准,其中以道地产区岷县当归药材质量较好。
Objective: To determine femlic acid content of DangGui from different habitats of Gansu by high performance liquid chromatography (HPLC) and to compare the quality of medicinal materials form different habitats objectively. Method: HPLC, the analytic column is ZORBAX Edipse XDB-C18 (250 min×4.6 mm, 5 μm), mobile phase:acetonitrile:0.085% water(17:83)(V/V); flow rate:l.0 mL/min; injection volume:10 μL; oven temperature:35℃; UV detection wavelength:316 nm. Result: Comparison of Ferulic acid contents of DangGui from three habitats showed significant difference, Minxian〉Zhangxian〉Hadapu, among them the highest ferulic acid content of DangGui from Minxian is 0.1206%. Ferulic acid contents of DangGui from three habitats are higher than the standard of Chinese traditional medicine Codex. Conclusion: Femlic acid contents of DangGui from three habitats are higher than the standard of Chinese traditional medicine Codex. Among them, DangGui of better quality is from Minxian.

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