文献类型：期刊文献

中文题名：基于脑肠轴理论探讨龙牡安神方对慢性束缚应激焦虑模型小鼠的影响

英文题名：The effect of the Longmu Anshen prescription on chronic restraint stress anxiety model mice based on brain-gut axis theory

作者：王伟龙[1];党心雨[1];刘立[2];朱鑫磊[1];李婷婷[1];彭晓明[1];侯悦晨[1];赵建军[1]

第一作者：王伟龙

机构：[1]甘肃中医药大学硕士研究生2020级,甘肃兰州730000;[2]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2022

卷号：14

期号：32

起止页码：39

中文期刊名：中医临床研究

外文期刊名：Clinical Journal Of Chinese Medicine

基金：自然科学基金(20JR10RA338)。

语种：中文

中文关键词：焦虑症;下丘脑;龙牡安神方;血管活性肽

外文关键词：Anxiety disorder;Hypothalamus;The Longmu Anshen prescription;Vasoactive peptide

摘要：目的:探讨不同剂量龙牡安神方对慢性束缚应激模型小鼠下丘脑、结肠中P物质和血管活性肽含量的影响。方法:将36只小鼠随机分为空白对照组6只和模型组30只,模型组小鼠接受21 d的慢性束缚应激方式处理,造模成功后随机分为模型对照组、龙牡安神方高、中、低剂量组和盐酸帕罗西汀组。龙牡安神方组和盐酸帕罗西汀组分别予以药物灌胃,21 d后处死小鼠后取下丘脑、海马、结肠等组织,检测炭沫在小鼠体内的小肠推进率和胃残留率,采用酶联免疫吸附测定法测定小鼠海马组织5-羟色胺、去甲肾上腺素和下丘脑、结肠P物质和血管活性肽的含量。结果:模型对照组与空白对照组相比,小鼠5-羟色胺、去甲肾上腺素降低,下丘脑、结肠P物质含量增加,血管活性肽含量降低,小肠推进率均显著降低,胃残留率增加(P<0.01),表明小鼠焦虑模型造模成功;龙牡安神方高、中剂量组和盐酸帕罗西汀组与模型对照组相比,5-羟色胺、去甲肾上腺素、下丘脑、结肠P物质含量显著降低,血管活性肽含量增加(P<0.01)。结论:中高剂量的龙牡安神方通过提高焦虑小鼠单胺类递质的含量调节下丘脑及结肠P物质和血管活性肽的表达,从而达到改善焦虑状态及胃肠功能的作用。
Objective:To investigate the effects of different doses of the Longmu Anshen prescription(龙牡安神方)on the contents of substance P and vasoactive peptide in hypothalamus and colon of chronic restraint stress model mice.Methods:A total of 36 mice were randomly divided into the blank control group of 6 mice and the model without administration group of 30 mice.The mice in the model group were given chronic restraint stress for 21 days.After successful modeling,they were randomly divided into the model control group,the high dose group,the medium dose group,the low dose group of the Longmu Anshen prescription,and the paroxetine hydrochloride group.The Longmu Anshen prescription group and the paroxetine hydrochloride group were given medicines gavage respectively.After 21 days,the mice in the groups of the longmu Anshen prescnption and the paroxetine hydrochloride group were killed,and the hypothalamus,hippocampus,colon and other tissues were taken to detect the small intestinal propulsion rate and gastric residue rate of carbon foam in mice.The contents of 5-hydroxytryptamine,norepinephrine and hypothalamus,colon substance P and vasoactive peptide in hippocampus were measured by enzyme-linked immunosorbent assay.Results:Compared with the blank control group,the contents of 5-hydroxytryptamine and norepinephrine in the model group were decreased,the contents of substance P in hypothalamus and colon were increased,the content of vasoactive peptide was decreased,the propulsion rate of small intestine was decreased significantly,and the gastric residue rate was increased(P<0.01),indicating that the anxiety model of mice was successfully established.Compared with the model control group,the contents of 5-hydroxytryptamine,norepinephrine,hypothalamus,and colon substance P in the high dose group and medium dose group of the Longmu Anshen prescription and the paroxetine hydrochloride group were decreased significantly,and the content of vasoactive peptide was increased(P<0.01).Conclusion:Medium and high doses of the Longmu Anshen prescription can ameliorate anxiety state and gastrointestinal function by increasing the content of monoamine transmitters and regulating the expression of colon substance P and vasoactive peptide in hypothalamus of anxiety mice.