文献类型：期刊文献

英文题名：Integrative Transcriptome and Metabolome Analysis Reveals the Regulatory Networks and Key Biosynthetic Pathway Genes of Wild and Cultivated Gentiana macrophylla Pall

作者：Liu, Juanjuan[1,2];Zhang, Jialing[1];Chen, Yiyang[1,3];Li, Ke[1];Ma, Xiaohui[1,4];Zhang, Xiaobo[2];Jin, Ling[1,4,5,6]

第一作者：Liu, Juanjuan;刘家骏;刘建军

通信作者：Jin, L[1];Zhang, XB[2];Jin, L[3];Jin, L[4];Jin, L[5]

机构：[1]Gansu Univ Tradit Chinese Med, Sch Pharm, Lanzhou 730000, Peoples R China;[2]China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100700, Peoples R China;[3]Nanjing Univ Tradit Chinese Med, Sch Pharm, Nanjing 210023, Peoples R China;[4]Engn Res Ctr Evaluat Protect & Utilizat Rare Tradi, Lanzhou 730000, Peoples R China;[5]Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou 730000, Peoples R China;[6]Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Sch Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100700, Peoples R China;[3]corresponding author), Engn Res Ctr Evaluat Protect & Utilizat Rare Tradi, Lanzhou 730000, Peoples R China;[4]corresponding author), Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou 730000, Peoples R China;[5]corresponding author), Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：16

期号：3

外文期刊名：METABOLITES

收录：;Scopus(收录号：2-s2.0-105034308409);WOS:【SCI-EXPANDED(收录号:WOS:001727079900001)】；

基金：This work was funded by China Agriculture Research System of MOF and MARA (CARS-21), Gansu Province Science and Technology Plan Project: Major Science and Technology Special (23ZDFA013-1), Gansu Provincial Science and Technology Plan Project: Science and Technology Special Touring Special (23CXNA0042), Gansu Provincial Rare Traditional Chinese Medicine Resources Evaluation and Protection and Utilization Engineering Research Center Open Fund (GSZXZY202203), Innovation Center for Traditional Chinese Medicine Co-constructed by Gansu Province & MOE of PRC, Strategic Research and Consulting Project of China Academy of Engineering (GS2021ZDA06), Improvement of traditional Chinese medicine guarantee and innovation capabilities (State Administration of Traditional Chinese Medicine), Scientific and technological innovation project of China Academy of Chinese Medical (CI2024E003).

语种：英文

外文关键词：Gentiana macrophylla (Gentianaceae); wild; cultivated; multi-omics; Iridoids; medicinal value

摘要：Objectives: Gentiana macrophylla Pall. (Gentianaceae) is a medicinally important perennial herb. Iridoids are the main chemical constituents of G. macrophylla. The scarcity of the wild resource has led to increased attention to cultivated G. macrophylla. However, little is known about the metabolic differences and the regulatory mechanisms between cultivated and wild G. macrophylla. Methods: This study utilized untargeted metabolomics and transcriptomics to reveal differentially accumulated metabolites (DAMs) and differentially expressed genes (DEGs) between wild and cultivated. Results: The metabolomics profiling revealed 25,587 DAMs (p < 0.05) while the transcriptomic profiling identified 6830 DEGs. Analysis revealed that DEGs were predominantly enriched for processes associated with monoterpenoid biosynthesis and flavonoid biosynthesis. In addition, we word validated six DEGs involved in monoterpenoid biosynthesis and flavonoid biosynthesis by RT-qPCR. According to KEGG pathway analysis, 10HGO (8-hydroxygeraniol dehydrogenase) may be a key enzyme encoding secoiridoid biosynthesis. The comprehensive results of transcriptome and metabolomics analysis revealed significant correlation between metabolite content and gene expression, providing a foundation for further study the function of G. macrophylla Pall. and the regulation of biosynthesis of active components. Conclusions: These approaches aim to explore the consistency of medicinal quality between the two sources across different habitats and to develop cultivated gentian as a full substitute for its wild counterpart in medicinal value. This strategy will fundamentally alleviate the predatory harvesting pressure on wild resources, ease their depletion, provide a theoretical basis for further development and protection of wild species of G. macrophylla in the future.