文献类型：期刊文献

中文题名：当归贝母苦参颗粒质量标准研究

英文题名：Study on quality standard for Danggui Beimu Kushen Granules

作者：李长天[1,2];张珺[1];吴国泰[1,2];石晓峰[3];邵晶[1,2];李威[1];刘永琦[1]

第一作者：李长天

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省中药药理与毒理学重点实验室,兰州730000;[3]甘肃省医学科学研究院,兰州730070

第一机构：甘肃中医药大学

年份：2016

卷号：32

期号：12

起止页码：1785

中文期刊名：现代医药卫生

外文期刊名：Journal of Modern Medicine & Health

基金：甘肃省财政厅科研业务费资助项目(BH-2013-25)

语种：中文

中文关键词：当归;浙贝母;苦参;阿魏酸;苦参碱;色谱法,薄层;色谱法,高压液相

外文关键词：Angelica sinensis; Fritillaria thunbergⅡ; Sophora flavescens; ferulic acid; Matrine; Chromatography; thin layer; Chromatography; high pressure liquid;

摘要：目的建立当归贝母苦参颗粒（当归、浙贝母、苦参）的质量标准。方法采用薄层色谱法对制剂中当归、浙贝母、苦参进行定性鉴别,采用高效液相色谱法测定当归贝母苦参颗粒中含阿魏酸、苦参碱量。结果当归、浙贝母、苦参薄层色谱鉴别方法专属性强、重现性较好;阿魏酸在0.031 6-0.129 6μg范围内与峰面积呈良好线性关系（r=0.999 8）,平均回收率为101.620%,相对标准偏差（RSD）为1.070%;苦参碱在0.100 0-1.000 0μg范围内与峰面积呈良好的线性关系（r=0.999 6）,平均回收率为99.180%,RSD为2.040%。结论该法简便、灵敏、准确、重复性好,可作为当归贝母苦参颗粒的质量控制标准。
Objective To set up the quality standard for Danggui Beimu Kushen Granules. Methods TLC was applied to qualitatively identify Angelica sinensis,Fritillaria thunbergⅡ,Sophora flavescens and HPLC was applied to quantitatively determine ferulic acid and matrine in the preparation. Results The TLC identification method of Angelica sinensis,Fritillaria Thunberg Ⅱ and Sophora flavescens had strong specificity and good reproducibility; ferulic acid showed good linear relation in the range of 0.031 6-0.129 6 μg（r=0. 999 8）. The average recovery rate of ferulic acid was 101.620%,and RSD was 1.070%;matrine showed good linearity with the peak area in the range of 0.100 0-1.000 0 μg（r=0.999 6）. The average recovery rate was99.180% and RSD was 2.04%. Conclusion This methods are simple,sensitive and accurate with good reproducibility,and can be used as the quality control standard of Danggui Beimu Kushen Granules.