文献类型：期刊文献

英文题名：Resveratrol and genistein inhibition of rat isolated gastrointestinal contractions and related mechanisms

作者：Zhang, Li-Xue[1,2];Li, Hong-Fang[3];Wang, Long-De[4];Jin, Shan[1];Dou, Xing-Cheng[1];Tian, Zhi-Feng[5];Ma, Qin[3]

第一作者：Zhang, Li-Xue

通信作者：Li, HF[1]

机构：[1]Lanzhou Univ, Coll Basic Med, Dept Physiol, Lanzhou 730000, Gansu, Peoples R China;[2]Northwest Univ Nationalities, Coll Med, Lanzhou 730030, Gansu, Peoples R China;[3]Lanzhou Univ, Coll Basic Med, Dept Physiol, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Gansu, Peoples R China;[4]Gansu Chinese Med Coll, Affiliated Hosp, Lanzhou 730020, Gansu, Peoples R China;[5]Lanzhou Univ, Funct Lab Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：Lanzhou Univ, Coll Basic Med, Dept Physiol, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Coll Basic Med, Dept Physiol, Key Lab Preclin Study New Drugs Gansu Prov, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2014

卷号：20

期号：41

起止页码：15335

外文期刊名：WORLD JOURNAL OF GASTROENTEROLOGY

收录：;Scopus(收录号：2-s2.0-84912124640);WOS:【SCI-EXPANDED(收录号:WOS:000345115100034)】；

基金：Grants from the Administration of Lanzhou Scientific Technology, No. 2010-1-88; Gansu Natural Science Fund in China, No. 1010RJZA111; and Natural Science Foundation of China, No. 21272103 and No. 81360540

语种：英文

外文关键词：Ca2+ channel; Gastrointestinal; Motility; Phytoestrogen; Smooth muscle

摘要：AIM: To investigate the effects and underlying mechanisms of resveratrol and genistein on contractile responses of rat gastrointestinal smooth muscle. METHODS: Isolated strips of gastrointestinal smooth muscle from Spraque-Dawley rats were suspended in organ baths containing Kreb's solution, and the contractility of smooth muscles was measured before and after incubation with resveratrol and genistein, and the related mechanisms were studied by co-incubation with various inhibitors. RESULTS: Resveratrol and genistein dose-dependently decreased the resting tension, and also reduced the mean contractile amplitude of gastrointestinal smooth muscle. Estrogen receptor blockades (ICI 182780 and tamoxifen) failed to alter the inhibitory effects induced by resveratrol and genistein. However, their effects were attenuated by inhibitions of alpha-adrenergic receptor (phentolamine), nitric oxide synthase (levorotatoryNG- nitroarginine), ATP-sensitive potassium channels (glibenclamide), and cyclic adenosine monophosphate (SQ22536). In high K+/Ca2+-free Kreb's solution containing 0.01 mmol/L egtazic acid, resveratrol and genistein reduced the contractile responses of CaCl2, and shifted its cumulative concentration-response curves rightward. CONCLUSION: Resveratrol and genistein relax gastrointestinal smooth muscle via alpha-adrenergic receptors, nitric oxide and cyclic adenosine monophosphate pathways, ATP-sensitive potassium channels, and inhibition of L-type Ca2+ channels. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.