文献类型：期刊文献

英文题名：lncRNA-NEF regulates hepatic stellate cells proliferation, cell cycle, apoptosis and ECM synthesis through the ERK1/2/c-Fos axis

作者：Jia, Gang-gang[1,2];Lu, Li-xia[2];Li, Bin-[2];Li, Chu-yi[2];Zheng, Ying-[2];Zhang, Jiu-cong[2];He, Yu-jing[2];Xu-Shi[2];Yu, Xiao-hui[1,2]

第一作者：Jia, Gang-gang

通信作者：Yu, XH[1]

机构：[1]Gansu Univ Chinese Med, Lanzhou, Peoples R China;[2]940th Hosp Joint Serv Logist Support Force PLA, Dept Gastroenterol, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：444

期号：2

外文期刊名：EXPERIMENTAL CELL RESEARCH

收录：;Scopus(收录号：2-s2.0-85210759010);WOS:【SCI-EXPANDED(收录号:WOS:001372504600001)】；

语种：英文

外文关键词：lncRNA-NEF; Liver fibrosis; ERK1/2; c -Fos; Apoptosis; HSCs

摘要：In this study, we investigated the role of lncRNA-NEF in modulating hepatic stellate cell (HSC) activation, a key process in liver fibrosis. Using the GSE78160 dataset, we identified lncRNA-NEF as downregulated in liver cirrhosis patients. Gene Ontology and KEGG analyses implicated it in transcriptional regulation and cell cycle control. We established an activated HSC model with TGF-(31-treated LX-2 cells and employed RT-qPCR and Western blot to assess lncRNA-NEF and ERK1/2 expression. Lentiviral transfection was used to overexpress lncRNA-NEF in activated LX-2 cells, and its effects on proliferation, apoptosis, and cell cycle were evaluated using EdU staining, CCK-8, Annexin-V PE/7-AAD, TUNEL, and PI-FACS analysis. Overexpression of lncRNA-NEF led to reduced cell proliferation, increased apoptosis, and cell cycle arrest at the S and G2/M phases. We also observed a decrease in ERK1/2, c-Fos, Collagen I, alpha-SMA, and Bcl-2 expression, and an increase in Caspase-3 expression, as confirmed by Western blot. These results suggest that lncRNA-NEF regulates HSC activation via the ERK1/2/c-Fos axis, potentially offering a therapeutic target for antifibrotic drug development. Our findings provide a molecular basis for understanding the role of lncRNAs in liver fibrosis and highlight the potential of lncRNA-NEF as a novel antifibrotic target.