文献类型：期刊文献

中文题名：Baicalin extracted from Huangqin(Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma(Bcl-2)/Bcl-2-associated X protein and activating caspase-3 and caspase-9

英文题名：Baicalin extracted from Huangqin(Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma(Bcl-2)/Bcl-2-associated X protein and activating caspase-3 and caspase-9

作者：Wang Hongwei[1,2,7];Li Hailong[3,4,5];Chen Fengqin[6];Luo Jun[6];Gu Jing[6];Wang Huping[1];Wu Hongyan[7];Xu Yan[6]

第一作者：Wang Hongwei;王宏伟

通信作者：Xu, Y[1]

机构：[1]Clinical Laboratory, The Hospital Affiliatedto Gansu University of Chinese Medicine, Lanzhou 730000,China;[2]Key Laboratory for Transfer of Dunhuang Medicine atthe Provincial and Ministerial Level, Lanzhou 730000, China;[3]Department of Teaching and Research of Clinical Laboratory, School of Clinical Medicine, Gansu Universityof Chinese Medicine, Lanzhou 730000, China;[4]Clinical Laboratory, The Hospital Affiliated to Gansu University of ChineseMedicine, Lanzhou 730000, China;[5]Key Laboratory for Transfer of Dunhuang Medicine at the Provincial and MinisterialLevel, Lanzhou 730000, China;[6]Key Laboratoryfor Transfer of Dunhuang Medicine at the Provincia andMinisterial Level, Lanzhou 730000, China;[7]Gansu Provincial Key Labora-tory of Chinese Medicinal Herbs for Prescription, Innovationand Transformation, Gansu University of Chinese Medicine,Lanzhou 730000, China

第一机构：Clinical Laboratory, The Hospital Affiliatedto Gansu University of Chinese Medicine, Lanzhou 730000,China

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2017

卷号：37

期号：2

起止页码：229

中文期刊名：Journal of Traditional Chinese Medicine

外文期刊名：中医杂志（英文版）

收录：Scopus;CSCD:【CSCD2017_2018】；PubMed;

基金：Supported by the Natural Science Foundation of Gansu Province (No. 148RJZA073); the Health Industry Scientific Research Project of Gansu Province (No. GWGL 2013-40); and the Openning Plan Project of Key Laboratory for Transfer of Dunhuang Medicine at the Provincial and Ministerial Level (No. DHYX1213-008)

语种：英文

中文关键词：Baicalin；胃瘤；Apoptosis 淋巴瘤；B 房间；Bcl-2-associated X 蛋白质；Caspases；受动器

外文关键词：Baicalin; Stomach neoplasms; Apoptosis Lymphoma; B-Cell; Bcl-2-associated X protein; Caspases; effector;

摘要：OBJECTIVE:To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.METHODS:Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells.Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin.RESULTS:In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 μmol/L baicalin for 48 h, a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner.Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma(Bcl-2)-associated X protein and down-regulated the expression of Bcl-2 at both the m RNA and protein level.CONCLUSION:Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells.
OBJECTIVE：To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.METHODS：Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells.Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin.RESULTS：In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 μmol/L baicalin for 48 h, a 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner.Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma（Bcl-2）-associated X protein and down-regulated the expression of Bcl-2 at both the m RNA and protein level.CONCLUSION：Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells.