文献类型：期刊文献

英文题名：N-Succinyl-chitosan nanoparticles induced mitochondria-dependent apoptosis in K562

作者：Luo, Huiying[1,2];Su, He[3];Wang, Xiaopeng[3];Wang, Lijuan[1];Li, Juan[4]

第一作者：罗慧英;Luo, Huiying

通信作者：Luo, HY[1]

机构：[1]Gansu Coll Tradit Chinese Med, Dept Pharmacol, Lanzhou 730000, Peoples R China;[2]Key Lab Pharmacol & Toxicol Tradit Chinese Med Ga, Lanzhou 730000, Peoples R China;[3]Gansu Prov Hosp, Lanzhou 730000, Peoples R China;[4]Lanzhou Univ, Hosp 1, Ctr Mol Biol, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Coll Tradit Chinese Med, Dept Pharmacol, 35 Dingxi Rd, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2012

卷号：26

期号：4

起止页码：164

外文期刊名：MOLECULAR AND CELLULAR PROBES

收录：;Scopus(收录号：2-s2.0-84861812423);WOS:【SCI-EXPANDED(收录号:WOS:000305658700004)】；

语种：英文

外文关键词：N-Succinyl-chitosan nanoparticles (NSCNP); Apoptosis; Cytochrome c; Caspase; Bax/Bcl-2

摘要：N-Succinyl-chitosan nanoparticles (NSCNP) are synthetical nanoparticles derived from chitosan. In our previous study, we demonstrated that NSCNP could induce apoptosis in K562 cells, which were evidenced by decreasing cell zeta potential, disrupting the mitochondrial membrane potential, increasing ROS generation and Ca2+ concentration. Here, by western blot we revealed that apoptosis induced by NSCNP was associated with accumulation of cytochrome c in cytosol and elevating expression of apoptosis-promoted protein Bax while depressing expression of apoptosis-restrained protein Bcl-2 in time- and dose-dependence. Cell cycle blocking in G2/M phase was evidenced by kinetic cell cycle analysis. The activations of caspase-9 and 3, not caspase-8 were observed by fluorescent and colorimetric assays. Our data suggested that the apoptotic signals triggered by NSCNP were mediated mainly through the intrinsic mitochondria-dependent pathway. (C) 2012 Elsevier Ltd. All rights reserved.