文献类型：期刊文献

中文题名：针刺对单眼视觉剥夺大鼠图形视觉诱发电位的影响

英文题名：Effect of acupuncture on pattern-visual evoked potential in rats with monocular visual deprivation

作者：严兴科[1];董莉莉[1];刘安国[1];王军燕[1];马重兵[1];朱田田[1]

第一作者：严兴科

机构：[1]甘肃中医学院针灸推拿系

第一机构：甘肃中医药大学针灸推拿学院

年份：2013

卷号：33

期号：8

起止页码：721

中文期刊名：中国针灸

外文期刊名：Chinese Acupuncture & Moxibustion

收录：MEDLINE(收录号：24195215);CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；PubMed;

基金：国家自然科学基金项目:81260560

语种：中文

中文关键词：单眼视觉剥夺;图形视觉诱发电位;针刺

外文关键词：monocular visual deprivation; pattern visual evoked potential （P-VEP） ; acupuncture

摘要：目的:探讨针刺防治视觉剥夺效应的电生理学机制。方法:健康15d龄long Evans大鼠18只,随机分为正常组(N)、模型组(MD)和针刺治疗组(MDT),每组6只。后两组采用单侧眼睑缝合的方法建立剥夺性弱视动物模型。针刺治疗组选取"睛明""承泣""球后""攒竹"穴针刺,双侧交替使用,每日针刺一侧穴位,共14d。观察针刺对不同空间频率下视觉诱发电位的影响。正常组与模型组不做任何治疗干预。结果:在2×2、4×4、8×8三种不同空间频率(SF)下,与正常组比较,模型组出现明显的剥夺效应,P1峰潜时延迟(P<0.01),N1-P1波幅值降低(P<0.01);与模型组比较,针刺治疗组P1峰潜时显著提前(P<0.01),N1-P1波幅值升高(P<0.01),与正常组比较差异无统计学意义(P>0.05)。另外,正常组、针刺治疗组大鼠均在SF为4×4时N1-P1波幅值达到最高,在此空间频率下鼠眼的分辨能力最佳,表明这可能是大鼠视觉系统的最佳空间频率。结论:视觉系统在敏感期内具有明显的电生理可塑性,针刺治疗可调节视觉剥夺所导致的视反应的抑制与迟缓,从而拮抗剥夺效应。
Objective To explore electrophysiology mechanism of acupuncture for treatment and prevention of visual deprivation effect. Methods Eighteen healthy 15-day Evans rats were randomly divided into a normal group, a model group and an acupuncture group, 6 rats in each one. Deprivation amblyopia model was established by monocular eyelid suture in the model group and acupuncture group. Acupuncture was applied at ＂Jingming＂ （BL 1）, ＂Chengqi＂ （ST 1）, ＂Qiuhou＂ （EX-HN 7） and ＂Cuanzhu＂ （BL 2） in the acupuncture group. The bilateral aeupoints were selected alternately, one side for a day, and totally 14 days were required. The effect of acupunc- ture on visual evoked potential in different spatial frequencies was observed. Methods Under three different kinds of spatial frequencies of 2× 2, 4 × 4 and 8 × 8, compared with normal group, there was obvious visual deprivation effect in the model group where P1 peak latency was delayed （P〈0. 01） while NI-P1 amplitude value was de- creased （P〈0.01）. Compared with model group, P1 peak latency was obviously ahead of time （P〈0.01） while N1-P1 amplitude value was increased （P〈0.01） in the acupuncture group, there was no statistical significance compared with normal group （P〉0.05）. Under spatial frequency of 4 ×4, NI-P1 amplitude value was maximum in the normal group and acupuncture group. With this spatial frequency the rat＇s eye had best resolving ability, in- dicating it could be the best spatial frequency for rat visual system. Conclusion The visual system has obvious e- lectrophysiology plasticity in sensitive period. Acupuncture treatment could adjust visual deprivation-induced sup- pression and slow of visual response in order to antagonism deprivation effect.