文献类型：期刊文献

中文题名：大豆异黄酮对骨质疏松大鼠模型的作用探讨

英文题名：Effect of soybean isoflavones on osteoporosis rat models

作者：张广文[1];陈青海[1];宁学乾[1];曾昭洋[1]

第一作者：张广文

机构：[1]甘肃中医药大学附属医院关节外科,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2020

卷号：32

期号：12

起止页码：1397

中文期刊名：中国微生态学杂志

外文期刊名：Chinese Journal of Microecology

收录：CSTPCD;;CSCD:【CSCD_E2019_2020】；

语种：中文

中文关键词：大豆异黄酮;骨质疏松;肠道菌群

外文关键词：Soybean isoflavones;Osteoporosis;Gut microflora

摘要：目的研究大豆异黄酮促进骨质疏松大鼠骨形成的作用及其肠道微生态的变化。方法从60只SPF级SD雌性大鼠中随机挑选50只建立去卵巢骨质疏松大鼠模型,余下10只为假手术组。将建模成功的大鼠随机分为5组,模型组、大豆异黄酮高、中、低剂量组(320 mg/kg、160 mg/kg、80 mg/kg的大豆异黄酮)、阿仑膦酸钠组(1 mg/kg阿仑膦酸钠),每日1次,治疗10周。比较治疗前后大鼠骨密度、血清1型前胶原N端前肽(P1NP)、骨碱性磷酸酶(BALP)、血清骨钙素(BGP)水平和肠道微生物变化。结果治疗后,模型组大鼠骨密度、血清P1NP、BALP、BGP水平均低于假手术组(P<0.05),阿仑膦酸钠组和大豆异黄酮高、中、低剂量组均高于模型组(P<0.05)。治疗后,阿仑膦酸钠组和大豆异黄酮高、中、低剂量与模型组比较股骨组织病变减轻;厚壁菌门、梭菌纲、芽孢杆菌纲、毛螺菌科、乳杆菌科、普氏菌科、肠球菌科、毛螺菌属、乳杆菌属、罗氏菌属、布劳特氏菌属、粪球菌属、普氏菌属水平相对丰度模型组均低于假手术组(P<0.05),阿仑膦酸钠组和大豆异黄酮高、中、低剂量组均高于模型组(P<0.05)。拟杆菌门、变形菌门、拟杆菌纲、γ-变形菌纲、毛菌纲、拟杆菌科、肠杆菌科、拟杆菌属、别样棒菌属、肠杆菌属模型组均高于假手术组(P<0.05),阿林磷酸钠组和大豆异黄酮高、中、低剂量组均低于模型组(P<0.05)。LEfSe分析结果显示,与模型组比较,阿仑膦酸钠组和大豆异黄酮高、中、低剂量组治疗后丁酸球菌属、放线菌属、拟杆菌科、拟杆菌属水平降低,消化球菌科、韦荣氏菌科、普氏菌属水平升高。结论大豆异黄酮可提高骨密度,提高血清骨形成指标水平,促进骨质疏松大鼠骨形成,还可改善大鼠肠道菌群。
Objective To observe the effect of soybean isoflavones(SIs)on bone formation and the changes of intestinal microecology in osteoporosis rats.Methods Fifty out of sixty SPF grade SD female rats were randomly selected to establish the models of ovariectomized osteoporosis,with the remaining 10 as the sham operation group(1 mL/100 g saline).After 3 months the osteoporosis models were successfully established,they were randomly divided into the model group(saline 1 mL/100 g),SIs high dose(SIs 320 mg/kg)group,SIs medium dose(SIs 160 mg/kg)group,SIs low dose(SIs 80 mg/kg)group or alendronate sodium(AS)(1 mg/kg)group.The changes in rat bone mineral density,serum type 1 procollagen N-terminal propeptide(P1 NP),bone alkaline phosphatase(BALP),serum osteocalcin(BGP)and intestinal microbe before and after treatment were compared among groups.Results After treatment,the bone mineral density and serum levels of P1 NP,BALP,and BGP in the model group were lower than those in the sham operation group(P<0.05);those in the AS group and the three SIs groups were higher than in the model group(P<0.05).Compared with the model group,the femur tissue lesions reduced in the AS group and the three SIs groups.The relative abundances of Pachycephalospora,Clostridium,Bacillus,Trichosporaceae,Lactobacillus,Przemyceae,Enterococciaceae,Trichosporon,Lactobacillus,Rhodobacter,Brautella,Coprococcus and Prevotella in the model group were lower than in the sham operation group(P<0.05),and those in the AS group and the three SIs groups were higher than in the model group(P<0.05).The relative abundances of Bacteroides,Proteobacteria,Bacteroides,γ-Proteobacteria,Mucor,Bacteroides,Enterobacteriaceae,Bacteroidaceae,Corynebacterium and Enterobacter in the model group were higher than in the sham operation group(P<0.05),and those in the AS group and the three SIs groups were lower than the in model group(P<0.05).The results of LEfSe analysis showed that compared with the model group,Butyrococcus,Actinomycetes,Bacteroids and Bacteroidaceae in AS group and the three SIs groups were down-regulated,while Digestive Coccidiaceae,Veillonellaceae and Pruneella were up-regulated.Conclusion Soybean isoflavones can increase bone density and serum bone formation indexes,promote bone formation in rats with osteoporosis,reduce the pathological changes of rat femur tissues,and improve intestinal microecology of rats by increasing the growth of beneficial bacteria while inhibiting the growth of pathogenic bacteria.