文献类型：期刊文献

中文题名：不同补肾方法对骨折大鼠BMP-2表达的影响

英文题名：Effects of Different Kidney-nourishing Methods on Expression of BMP-2 of Rats with Bone Fracture

作者：徐克武[1];王长慧[1];强胜林[1];于海洋[1]

第一作者：徐克武

机构：[1]甘肃中医学院附属医院,甘肃兰州730020

第一机构：甘肃中医药大学第二附属医院

年份：2013

卷号：26

期号：6

起止页码：15

中文期刊名：西部中医药

外文期刊名：Western Journal of Traditional Chinese Medicine

收录：CSTPCD

语种：中文

中文关键词：骨折;中药疗法;BMP-2;动物实验

外文关键词：bone fi＇acture; TCM therapy; BMP-2; animal experiment

摘要：目的:研究不同补肾方法对骨头形态发生蛋白-2(BMP-2)在骨折愈合过程中表达的影响。方法:于SD大鼠左胫骨上1/3段造成骨折,对骨折不做任何固定。随机分为生理盐水组、左归丸组、右归丸组、补肾壮筋汤组,每组9只。于1、2、4周时每组处死3只,取骨痂标本,免疫组化法检测标本中BMP-2水平,应用计算机图像分析系统进行定量分析。结果:补肾壮筋汤组BMP-2表达阳性细胞平均光密度(MOD)值显著高于左归丸组、右归丸组及生理盐水组(P<0.05)。结论:补肾壮筋汤能显著促进骨折愈合过程中BMP-2的表达。
Objective： To study the effects of different kidney-nourishing methods on the expression of BMP-2 in the course of bone healing. Method： Rat models with bone fracture were established at the place of 1/3 of left tibia and they were unhandled. The rats were randomized into four groups： physiological saline group, ZuoGuiWan group,YouGuiWan group and BuShen ZhuangJinTang group, nine rats each group. Every three rats of each group were sac- rificed at the 1 st, 2nd and 4th week, callus specimens were taken and BMP-2 in the samples were tested with im- munohistochemical assay, quantitative analysis was performed with computer imaging system. Result： Average opti- cal density （MOD） values of BMP-2 positive cells in BuShen ZhuangJinTang group were higher than that of ZuoGui Wan group, YouGuiWan group and physiological saline group （P〈0.05）. Conclusion： BuShen ZhuangJinTang could significantly promote the expressions of BMP-2 during the healing process