文献类型：期刊文献

英文题名：Autotoxic compounds from rhizosphere soil of Lanzhou lily extracts: Identification and biological activity

作者：Huang, Y. F.[1];Zhang, E. H.[2];Zhang, X. H.[3];Wang, Q.[2];Wang, H. Z.;Liu, Q. L.[2];Yuan, J. L.;Cui, J. J.[2]

第一作者：黄钰芳

通信作者：Huang, YF[1]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China; Northwest Collaborat Innovat Ctr Tradit Chinese M, Lanzhou 730000, Peoples R China;[2]Gansu Agr Univ, Agron Coll, Lanzhou 730070, Peoples R China;[3]Ningxia Med Univ, Coll Pharm, Yinchuan 750004, Ningxia, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2021

卷号：53

期号：1

起止页码：111

外文期刊名：ALLELOPATHY JOURNAL

收录：;Scopus(收录号：2-s2.0-85105953948);WOS:【SCI-EXPANDED(收录号:WOS:000638230000010)】；

基金：This research was supported by the Innovation Ability Promotion Project of Colleges and Universities of Gansu Province (2019A-072), the National Natural Science Foundation of China (31560380), and Gansu Agricultural University Campus Project (GSAU-ZL2015-007).

语种：英文

外文关键词：allelochemicals; Autotoxic compounds; autotoxicity; antioxidant 2246; dioctyl terephthalater; bioassay; bioactivity; GC-MS; lanzhou lily; Lilium davidii var. unicolor; pot culture; rhizosphere soil

摘要：We investigated the autotoxicity of Lanzhou lily rhizosphere soil with different cultivation years. The allelochemicals in such soils were isolated and identified by gas chromatography-mass spectrometry (GC-MS). Based on our earlier studies, the antioxidant 2246 and dioctyl terephthalate were found in lily sick soil were used in pot experiments determine to their autotoxic effects on the Lanzhou lily seedlings growth, photosynthetic parameters and antioxidant enzyme activities in seedling leaves. The content of antioxidant 2246 and dioctyl terephthalate was determined by GC-MS in rhizosphere soil of different cultivation years. The aqueous extracts of Lanzhou lily rhizosphere soil promoted the growth of its own seedlings at 0.2 mg.mL(-1), however, the concentrations > 2 mg.mL(-1) were inhibitory. The longer the cultivation period (1-yr, 2-yr and 4-yr), the stronger were the inhibitory effects. In rhizosphere soils of 1-yr, 2-yr and 4-yr; 8,: 15 and 18 compounds were identified, respectively. The identified compounds were camphor, 3-hydroxy-4-methoxy-benzaldehyde, 2,4-bis(1,1-dimethylethyl)-phenol, tributyl phosphate, dibutyl phthalate, antioxidant 2246 and dioctyl terephthalater and these are reported as allelochemicals. The pot experiment results showed that low concentrations of antioxidant 2246 stimulated the seedling growth but high concentrations were inhibitory, while all concentrations of dioctyl terephthalater inhibited the seedling growth. At 100 mg.mL(-1), the antioxidant 2246 and dioctyl terephthalate significantly inhibited the photosynthesis and antioxidant enzyme activity in leaves (P < 0.05). Furthermore, the content of these two compounds in soils increased with the increase of cultivation years. These results suggested that allelochemicals accumulated in replanted soil contributed to the autotoxicity of Lanzhou lily in rhizosphere soil.