文献类型：期刊文献

中文题名：复方地黄颗粒对帕金森病阴虚动风证6-OHDA模型大鼠黑质α-syn、VAPB、PTPIP51表达的影响

英文题名：Effects of Compound Dihuang Granules on α-syn, VAPB, PTPIP51 on substantia nigra of 6-hydroxydopamine-induced Parkinson’s disease model rats with Yin deficiency and wind syndrome

作者：孙光洁[1];王行玲[1];吕子微[1];何建成[2];梁建庆[1]

第一作者：孙光洁

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730000;[2]上海中医药大学基础医学院,上海201203

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2024

卷号：40

期号：6

起止页码：1179

中文期刊名：中国药理学通报

外文期刊名：Chinese Pharmacological Bulletin

收录：CSTPCD;;Scopus;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金资助项目(No 82160929);2021年度甘肃省科技厅第十一批省级科技计划自然科学基金(自筹经费)项目(No 21JR11RA143);敦煌医学与转化教育部重点实验室开放课题(No DHYX18-08);2022年度中医学一级学科“岐黄英才”导师专项基金硕导项目(No ZYXKSD-202211)。

语种：中文

中文关键词：帕金森病;阴虚动风证;复方地黄颗粒;α-syn;VAPB;PTPIP51

外文关键词：Parkinson’s disease;Yin deficiency and dynamic wind syndrome;compound Dihuang granules;α-syn;VAPB;PTPIP51

摘要：目的观察复方地黄颗粒对帕金森病(Parkinson’s disease,PD)大鼠黑质α-syn、VAPB、PTPIP51表达的影响,并探讨其潜在作用机制。方法构建6-羟基多巴胺(6-OHDA)PD大鼠模型,成模大鼠随机分为Model组、Madopar组、CLD组、CMD组、CHD组,NC组不做任何处理,SO组注射抗坏血酸,每组13只。检测大鼠神经行为学变化;Western blot、RT-PCR、免疫组化检测黑质α-syn、VAPB、PTPIP51表达;HE染色观察黑质组织病理形态变化;透射电镜观察黑质细胞线粒体超微结构;ELISA检测各组黑质组织ATP含量变化。结果与NC组和SO组比,Model组大鼠旋转圈数和爬杆时间增加,α-syn表达升高,VAPB、PTPIP51表达降低,神经元细胞数量减少、胞体皱缩,线粒体肿胀,线粒体嵴消失,ER-线粒体接触距离变大,ATP含量降低;与Model组相比,Madopar组和CLD组、CMD组、CHD组大鼠旋转圈数和爬杆时间减少,α-syn表达降低,VAPB、PTPIP51表达升高,神经元损伤程度减轻,线粒体形态有所改善,ATP含量增加,呈量效差异性,Madopar组和CHD组相对疗效较佳且差异无统计学意义。结论复方地黄颗粒能够减轻PD大鼠行为症状,并可能通过下调α-syn表达,上调PTPIP51、VAPB表达,改善线粒体功能,从而发挥治疗PD的作用。
Aim To observe the effects of Compound Dihuang Granules onα-syn,VAPB and PTPIP51 in the substantia nigra of Parkinson’s disease(PD)rats with Yin deficiency and wind syndrome,and to explore the possible mechanisms of their actions.Methods The 6-hydroxydopamine-induced PD model of rats was constructed.The model rats were randomly divided into the model group,madopar group,CLD group,CMD group and CHD group,while the NC group did not receive any treatment and the SO group was injected with ascorbic acid,with 13 rats in each group.The neurobehavioral changes of the rats were observed,and the expressions ofα-syn,VAPB and PTPIP51 in the substantia nigra were detected by Western blot,RT-PCR and Immunohistochemistry;the histopathological and morphological changes of the substantia nigra tissue were observed by HE staining,the changes of mitochondrial ultrastructure in the substantia nigra cells were observed by transmission electron microscopy,and the changes of ATP content in substantia nigra tissue in each group were detected by ELISA.Results Compared with the NC and SO groups,rats in the model group showed that the number of rotational circles and pole-climbing time increased,the expression ofα-syn increased,the expression of VAPB and PTPIP51 decreased,the number of neuronal cells decreased,the neuronal cells became crumpling,and mitochondrial swelling,disappearance of the mitochondrial cristae,a larger distance between the ER-mitochondrial contacts were observed;the ATP content decreased.Compared with the model group,rats in Madopar group and CLD group,CMD group and CHD group showed that the number of rotational circles and pole-climbing time decreased,the expression ofα-syn decreased,the expression of VAPB and PTPIP51 increased,the degree of neuronal damage was reduced,the morphology of mitochondria was improved and the content of ATP increased,showing the change of the difference in quantitative efficacy;the relative efficacy of Madopar group and CHD group was better,and there was no statistically significant difference.Conclusions Compound Dihuang granules attenuate the behavioral symptoms in PD rats and may play a therapeutic role in PD by down-regulating the expression ofα-syn,up-regulating the expression of PTPIP51 and VAPB and improving mitochondrial function.