文献类型：期刊文献

英文题名：One bioprobe: a fluorescent and AIE-active macromolecule; two targets: nucleolus and mitochondria with long term tracking

作者：Ma, Hengchang[1];Yang, Zengming[1];Cao, Haiying[1];Lei, Lei[1];Chang, Lu[1];Ma, Yucheng[1];Yang, Manyi[1];Yao, Xiaoqiang[1];Sun, Shaobo[2];Lei, Ziqiang[1]

第一作者：Ma, Hengchang

通信作者：Ma, HC[1];Lei, ZQ[1]

机构：[1]Northwest Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Eco Environm Related Polymer Mat, Lanzhou 730070, Peoples R China;[2]Gansu Univ Chinese Med, Inst Integrated Tradit & Western Med, Lanzhou 730070, Peoples R China

第一机构：Northwest Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Eco Environm Related Polymer Mat, Lanzhou 730070, Peoples R China

通信机构：[1]corresponding author), Northwest Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Eco Environm Related Polymer Mat, Lanzhou 730070, Peoples R China.

年份：2017

卷号：5

期号：4

起止页码：655

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20170503301973);Scopus(收录号：2-s2.0-85010679760);WOS:【SCI-EXPANDED(收录号:WOS:000395419800002)】；

基金：We acknowledge support from the National Natural Science Foundation of China (No. 21174114, 21361023) and the Ministry of Education Scholars Innovation Team (IRT 1177).

语种：英文

外文关键词：Cytology - Mitochondria - Target tracking - Probes - Bioimaging - Fluorescence

摘要：Specific organelle imaging and long-term cellular tracking are of paramount importance in monitoring biological processes, pathological pathways, and therapeutic effects, etc. Herein, we report a novel macromolecule fluorescent probe (TPPA-DBO), which is synthesized from tris(4-(pyridin-4-yl) phenyl) amine (TPPA) and 1,8-dibromononane (DBO) with a gram scale by a simple method. TPPA-DBO demonstrates a highly specific nucleolus-targeting ability, which is very challenging in the bioimaging research field. We have shown that the green nucleolus-specificity probe TPPA-DBO has advantages over the commercially available nucleolus-staining probes such as DAPI, Hoechst dyes and SYTOs in terms of its AIE-performance, large Stokes shift (175 nm), excellent photostability, and promising usefulness in live cell imaging experiments. Surprisingly, after internalizing TPPA-DBO into the nucleus region for a period of time, some TPPA-DBO are reversely diffused from nucleolus into the cytoplasm, thus resulting in the staining of mitochondria with a redder emission color. This research result may provide a new concept of cellular tracker design and provide insight into biological questions, understanding disease mechanismss, and designing new therapeutic strategies.