文献类型：期刊文献

中文题名：基于熵权TOPSIS模型的桃儿七种子超低温保存条件筛选

英文题名：Selection of Cryopreservation Conditions of Sinopodophyllum hexandrum Seeds Based on Entropy Weight TOPSIS Model

作者：久西加[1];王玉辉[1];陈红刚[1,2];王惠珍[1,2];曾翠云[1,2];杜弢[1,2]

第一作者：久西加

机构：[1]甘肃中医药大学和政药用植物园,和政731200;[2]西北中藏药协同创新中心,兰州730000

第一机构：甘肃中医药大学

年份：2023

卷号：43

期号：3

起止页码：404

中文期刊名：植物研究

外文期刊名：Bulletin of Botanical Research

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

基金：国家中药材产业技术建设专项资金资助(CARS-21);甘肃省科技创新基地和人才计划项目资助(18JR2TA017)。

语种：中文

中文关键词：超低温保存;桃儿七种子;含水量;冷冻方式;生理生化;

外文关键词：cryopreservation;Sinopodophyllum hexandrum seed;water content;freezing method;physiological and biochemical characteristics

摘要：探索适宜桃儿七(Sinopodophyllum hexandrum)种子超低温保存的条件,为桃儿七种子的长期保存提供技术支撑。以桃儿七种子为试验材料,利用硅胶干燥法、自然阴干法及浸泡法获取1.6%、9.6%、20.9%、30.5%的4种不同含水量种子,并通过直接冷冻、缓慢冷冻及玻璃化冷冻3种方式对其进行超低温保存,同时测定超低温保存前后桃儿七种子生活力及其种子内超氧化物歧化酶(SOD)、过氧化物酶(POD)、丙二醛(MDA)、总蛋白(TP)、可溶性淀粉、可溶性糖的含量变化。研究结果发现:(1)不同含水量的桃儿七种子经3种超低温冷冻方式保存后,种子生活力均呈现不同程度的下降,但含水量为1.6%、9.6%和20.9%的桃儿七种子经缓慢冷冻后较对照组无显著差异(P>0.05);(2)4种含水量的桃儿七种子经超低温保存后,其种子内可溶性糖和总蛋白含量总体呈上升趋势,且可溶性糖含量增幅最大,而种子内淀粉含量、超氧化物歧化酶和过氧化物酶活性总体呈下降趋势,但超氧化物歧化酶和过氧化物酶活性在含水量为1.6%和9.6%时降幅较小,丙二醛含量在含水量为1.6%和30.5%时呈上升趋势,而在含水量为9.6%和20.9%时表现为下降趋势,说明不同含水量的桃儿七种子经液氮冷冻后均受到不同程度损伤,引起抗氧化酶活性下降,同时蛋白含量的增加和淀粉降解导致的可溶性糖含量积累又提高了种子的低温耐性,导致冷害产物丙二醛含量呈现不同的变化趋势;(3)熵权TOPSIS综合排序在前3位的分别是含水量为1.6%的玻璃化冷冻组种子、含水量为9.6%的直接冷冻组和缓慢冷冻组的种子。结果表明,桃儿七种子液氮超低温保存技术是可行的,含水量为9.6%的种子经直接冷冻或缓慢冷冻为最佳的超低温保存条件。
To explore the optimal cryopreservation conditions for Sinopodophyllum hexandrum seeds,and to provide technical support for the long-term preservation of S.hexandrum seeds,four kinds of seeds with different moisture contents(1.6%,9.6%,20.9%and 30.5%)were obtained by silica gel drying,natural shade drying and soaking methods,and cryopreserved by direct freezing,slow freezing and vitrification respectively,and the seed viability and the activity of superoxide dismutase(SOD),peroxidase(POD),and the contents of malondialdehyde(MDA),total protein(TP),soluble starch and soluble sugar in seeds were measured before and after cryopreservation respectively.The results showed that the seed viability of S.hexandrum seeds with different water content declined in varying degrees after three cryopreservation methods,but the seeds with water content of 1.6%,9.6%and 20.9%had no significant difference compared with the control group(P0.05).After cryopreservation,the contents of soluble sugar and total protein in S.hexandrum seeds with four kinds of water content increased,and the content of soluble sugar was the biggest,while the content of starch in seeds showed a downward trend as a whole,and the activities of superoxide dismutase and peroxidase declined slightly when the water content was 1.6%and 9.6%.The content of MDA increased when the water content was 1.6%and 30.5%,but decreased when the water content was 9.6%and 20.9%,indicating that the seeds with different water content were damaged to different degrees after freezing in liquid nitrogen.The activities of antioxidant enzymes decreased,while the increase of protein content and the accumulation of soluble sugar content caused by starch degradation improved the low temperature tolerance of seeds.As a result,the content of malondialdehyde(MDA)in chilling injury products showed different trends.The seeds of vitrification freezing group with 1.6%water content and seeds of direct freezing group and slow freezing group with 9.6%water content were the top three places by entropy weight TOPSIS.The results showed that the liquid nitrogen cryopreservation technology of S.hexandrum seeds could be feasible,and the seeds with 9.6%water content would be the best cryopreservation conditions by direct freezing or slow freezing.