文献类型：期刊文献

中文题名：RP-HPLC法测定展筋酊中红花黄色素A的含量

英文题名：Determination of the Content of Safflor Yellow A in Zhanjin Tinctura by RP-HPLC

作者：王谨慧[1];靳子明[1];杨锡仓[1]

第一作者：王谨慧

机构：[1]甘肃中医学院附属医院,兰州730020

第一机构：甘肃中医药大学第二附属医院

年份：2008

卷号：19

期号：6

起止页码：480

中文期刊名：中药新药与临床药理

外文期刊名：Traditional Chinese Drug Research and Clinical Pharmacology

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：甘肃省科学事业费资助项目(GS061-C33-29)。

语种：中文

中文关键词：展筋酊;红花黄色素A;含量测定;HPLC

外文关键词：Zhanjing Tinctura ; Safflor yellow A ; Content determination; HPLC

摘要：目的建立展筋酊中红花黄色素A的HPLC测定方法。方法SymmetryC18色谱柱(4.6mm×150mm,5.0μm),流动相为甲醇-水-冰醋酸(7∶93∶2),pH=2.80,流速1.0mL·min-1,柱温为20℃,检测波长为402nm。结果红花黄色素A进样量在0.096～0.960μg范围内与峰面积积分值线性关系良好,r=0.9999;平均加样回收率为98.80%,RSD=2.85%(n=9)。结论该方法灵敏、准确、快速、专属性强,可用于展筋酊的含量测定。
Objective To establish an RP- HPLC method for the determination of the content of safflor yellow A in Zhanjing Tinctura. Methods The chromatographic procedure was carried out in Symmetry C18 column（4. 6 mm × 150 mm, 5.0 μm）with methanol - water - glacial acetic acid（7 ： 93 ： 2）as the mobile phase, pH 2.80. The flow rate was 1.0 mL · min^- 1, column temperature was 20 ℃ , and the detection wavelength was 402 nm. Results Safflor yellow A was well separated from other components. The standard curves of safflor yellow A showed linearity in the range of 0. 096 0. 960 μg （ r = 0. 999 9）. The average recovery was 98.80 % with RSD being 2.95 % （ n = 9）. Conclusion This method is sensitive, simple and accurate, and can be used for the determination of safflor yellow A in Zhanjing Tinctura.