文献类型：期刊文献

中文题名：两类根际复配促生菌剂对当归生长、生理特征及药效成分的影响

英文题名：Effect of Two Kinds of Compound Rhizosphere Growth-promoting Bacteria on Growth,Physiological Characteristics and Pharmacodynamic Components of Angelicae Sinensis Radix

作者：姚阳阳[1];杨涛[2];王引权[1,3];彭桐[1];王治业[2];杨晖[2]

第一作者：姚阳阳

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃省科学院生物研究所,甘肃省微生物资源开发利用重点实验室,兰州730000;[3]西北中藏药协同创新中心,甘肃省中药质量与标准研究重点实验室培育基地,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2022

卷号：28

期号：8

起止页码：131

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家重点研发计划项目(2017YFC1700705);甘肃省教育厅双一流重大科研项目(GSSYLXM-05);甘肃省重点研发计划项目(21YF1FA353);甘肃省科学院产业化基金项目(CY02);甘肃中医药大学扶贫科技专项(2019FPZX-1)。

语种：中文

中文关键词：当归;促生菌;假单胞菌属;芽孢杆菌属;光合作用;内源激素;药效成分

外文关键词：Angelicae Sinensis Radix;plant growth-promoting rhizobacteria;Pseudomonas;Bacillus;photosynthesis;endogenous hormones;pharmacodynamic composition

摘要：目的:研究叶面喷施两类根际复配促生菌剂对当归生长与生理特征、药效成分的影响,以期为当归生态种植提供功能性微生物菌剂奠定基础。方法:在田间叶面喷施浓度为1×10^(8)CFU·m L^(-1)的复配促生菌剂T1(假单胞菌属CBS5、CBS7和CBSB)和T2(芽孢杆菌属5C1、5C5和5C7),以喷施无菌马铃薯葡萄糖肉汤培养基为对照(CK)。常规方法测定当归植株生长指标,利用便携式光合测量系统测定当归植株光合生理指标,试剂盒法测定植物和微生物相关酶活性,超高效液相色谱串联质谱法分析植物内源激素水平,高效液相色普法测定阿魏酸、洋川芎内酯I、阿魏酸松柏酯、洋川芎内酯A、Z-藁本内酯含量。结果:与CK比较,2种复合菌剂能促进当归植株生长,增加生物量;提高叶片净光合速率、气孔导度、胞间CO_(2)浓度、蒸腾速率,增加过氧化氢酶、过氧化物酶、超氧化物歧化酶、多胺氧化酶、二胺氧化酶和多酚氧化酶的活性,增加植株中内源茉莉酸、细胞分裂素及赤霉素水平,提高当归药材阿魏酸、洋川芎内酯A、Z-藁本内酯含量;降低膜脂过氧化产物丙二醛和衰老因子脱落酸的含量;减少根腐发病率。结论:叶面喷施两类根际复配促生菌剂对当归生长、光合作用和抗胁迫能力均有促进作用,对当归药材品质形成有不同程度的提升。综合分析,当归叶面喷施复合菌剂T1处理的促生增质作用优于T2处理。
Objective:To study the effects of foliar spraying of two kinds of compound rhizosphere growth-promoting agents on the growth and physiological characteristics of Angelicae Sinensis Radix(ASR),as well as the pharmacodynamic components,in order to lay a foundation for providing functional microbial agents for ecological cultivation of ASR.Method:The compound growth-promoting agents T1(Pseudomonas CBS5,CBS7 and CBSB)and T2(Bacillus 5C1,5C5 and 5C7)with the concentration of 1×10^(8)CFU·m L^(-1)were sprayed on the leaf surface of the field,and the sterile potato glucose broth medium was used as the control(CK).The plant growth indexes of ASR were measured by conventional methods,the photosynthetic physiological indexes of ASR were measured by portable photosynthetic measurement system,the enzyme activities of plants and microorganisms were measured by kit method,and the endogenous hormone levels were analyzed by ultra-performance liquid chromatography tandem mass spectrometry.The contents of ferulic acid,senkyunolide I,coniferyl ferulate,senkyunolide A and Z-ligustilide were determined by high performance liquid chromatography.Result:Compared with CK,the two compound inoculants could promote the growth of ASRand increase the biomass,increase the leaf net photosynthetic rate,stomatal conductance,intercellular CO_(2)concentration,transpiration rate,increase catalase,peroxidase,superoxide dismutase,polyamine oxidase,diamine oxidase and polyphenol oxidase enzyme activities,increase endogenous jasmonic acid,cytokinin and gibberellin levels in plants,increase the contents of ferulic acid,senkyunolide A and Z-ligustilide,reduce the contents of malondialdehyde and abscisic acid,and reduce the incidence of root rot.Conclusion:Foliar spraying of two kinds of rhizosphere compound growth-promoting agents can promote the growth,photosynthesis and stress resistance of ASR,and can improve the quality of ASR in different degrees.Comprehensive analysis shows that T1 treatment is better than T2 treatment in the growth-promoting and qualityenhancing of ASR.