文献类型：期刊文献

中文题名：四逆散冻干粉改善睡眠作用的药效物质基础

英文题名：Pharmacodynamic material basis of Sinipowder for improving sleep

作者：李越峰[1,2];苏云明[2];严兴科[1];李廷利[3]

第一作者：李越峰

机构：[1]甘肃中医学院药学系,甘肃兰州730000;[2]黑龙江中医药大学博士后流动站,黑龙江哈尔滨150040;[3]黑龙江中医药大学药学院,黑龙江哈尔滨150040

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2013

卷号：27

期号：1

起止页码：18

中文期刊名：中国药理学与毒理学杂志

外文期刊名：Chinese Journal of Pharmacology and Toxicology

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金(81073077);教育部科学技术研究重点项目(212186);甘肃省自然科学基金(1010RJZA212)~~

语种：中文

中文关键词：四逆散;血清;脑脊液;睡眠;有效成分

外文关键词：Sini powder; blood serum; cerebrospinal fluid; sleep; effective constituent

摘要：目的探讨四逆散改善睡眠作用的药效物质基础,为阐明其改善睡眠的作用机制提供依据。方法 Wistar大鼠随机分为正常对照、四逆散冻干粉12 g·kg-1、芍药苷87 mg·kg-1、甘草次酸39 mg·kg-1、昔奈福林38 mg·kg-1、柴胡皂苷C 42 mg·kg-1、血清移行成分配伍(昔奈福林∶芍药苷∶柴胡皂苷C∶甘草次酸=8.5∶1∶1.5∶6.5)206 mg·kg-1组,给药组ig给药,每天1次,连续7 d,于末次给药后5 h制备血清或抽取脑脊液,用HPLC测定血清和脑脊液中四逆散移行成分;或者连续ig给药7 d后,ip给予戊巴比妥钠50 mg·kg-1,用翻正反射法测定小鼠睡眠时间。结果①四逆散冻干粉连续给药7 d后血清样品中有14种移行成分,经来源认定研究认为,其中2号成分为昔奈福林,4号成分为芍药苷,12号成分为柴胡皂苷C,14号成分为甘草次酸。②四逆散冻干粉、芍药苷、甘草次酸、昔奈福林和柴胡皂苷C均可以使脑脊液中内源性物质峰面积增加,其中四逆散冻干粉组是正常脑脊液峰面积的12.5倍;血清移行成分配伍组脑脊液中内源性物质峰面积高于四逆散冻干粉组,是四逆散冻干粉组的3.2倍。③昔奈福林、芍药苷、柴胡皂苷C和甘草次酸单用对戊巴比妥钠所致小鼠睡眠时间无明显影响,四逆散冻干粉和血清移行成分配伍均延长小鼠睡眠时间(P<0.01),且血清移行成分配伍组优于四逆散冻干粉组(P<0.01)。结论芍药苷、甘草次酸、昔奈福林和柴胡皂苷C是四逆散冻干粉给药后血清主要移行成分,可能是四逆散改善睡眠作用的物质基础。
OBJECTIVE To explore effective constituents in Sini powder （SP） that are sedative- hypnotic. METHODS Wistar rats were randomly divided into normal control, freeze-dried SP 12 g· kg^-1, paeoniflorin 87 mg· kg^-1 , glycyrrhetinic acid 39 mg· kg^-1 , synephrine 38 mg· kg^-1, saikosa- ponin C 42 mg· kg^-1 and prescription of SP serum transitional ingredients （PSTI） （synephrine： paeoniflor- in： saikosaponin C： glycyrrhetinic acid =8.5： 1：1.5：6.5） 206 mg· kg^-1 groups. The rats were ig given the corresponding drug once a day for seven consecutive days. The serum was prepared or cerebrospinal fluid was extracted 5 h after the last drug administration. Transitional ingredients of SP in blood and cere- brospinal fluid were analyzed by using HPLC method. In addition, the mice were ig given the corre- sponding drug once a day for seven days, and the sleep time induced by pentobarbital sodium was measured using righting reflex test. RESULTS （1） After the rats were ig given SP for seven days, there were 14 kinds of transitional ingredients in the serum. The 2nd ingredient was synephrine, the 4th one was paeoniflorin , the 12th one was saikosaponin C and the 14th one was glycyrrhetinic acid. （2） SP, paeoniflorin, glycyrrhetinic acid, synephrineand and saikosaponin C could make the peak area of the en- dogenous substance in cerebrospinal fluid larger than that in normal cerebrospinal fluid. SP group was 12.5 times the peak area of normal group. In addition, the peak area of the endogenous substance in cerebrospinal fluid of PSTI group was 3.2 times that of SP group. （3） Synephrine, paeoniflorin, saikosa- ponin C and glycyrrhetinic acid had no obvious effect on the sleeping time of mice induced by pentobarbi- tal sodium compared with normal cOntrol group. SP and PSTI could prolong the mouse sleeping time （ P 〈0.01 ）, expecially PSTI （ P 〈0.01 ）. CONCLUSION Paeoniflorin, glycyrrhetinic acid, synephrine and saikosaponin C are the serum transitional components of SP, which may be the effective constitu- ents in SP that can improve the sedative function.