文献类型：期刊文献

英文题名：Fecal Microbiota Transplantation Regulates Blood Pressure by Altering Gut Microbiota Composition and Intestinal Mucosal Barrier Function in Spontaneously Hypertensive Rats

作者：Xu, Xinghua[1,2];Jin, Hua[1];Li, Xiaoling[2];Yan, Chunlu[1];Zhang, Qiuju[1];Yu, Xiaoying[3];Liu, Zhijun[4];Liu, Shuangfang[1];Zhu, Feifei[5]

第一作者：Xu, Xinghua

通信作者：Jin, H[1]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[2]Lanzhou Univ, Hosp 2, Lanzhou 730030, Gansu, Peoples R China;[3]Gansu Prov Hosp Tradit Chinese Med, Lanzhou 730020, Peoples R China;[4]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou 730020, Peoples R China;[5]Tianshui Municipal Hosp Tradit Chinese Med, Tianshui 741000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

外文期刊名：PROBIOTICS AND ANTIMICROBIAL PROTEINS

收录：;Scopus(收录号：2-s2.0-85201798282);WOS:【SCI-EXPANDED(收录号:WOS:001296520800001)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 81560716 and 81860832). DAS:The data underlying this article will be shared on reasonable request to the author.

语种：英文

外文关键词：Fecal microbiota transplantation; Gut microbiota; Hypertension; Intestinal mucosal barrier; Spontaneously hypertensive rats

摘要：Hypertension is accompanied by gut microbiota imbalance, but the role of bacteria in the pathogenesis of hypertension requires further study. In this study, we used fecal microbiota transplantation to determine the impact of microbiota composition on blood pressure in spontaneous hypertensive rats (SHRs), using normotensive Wistar Kyoto (WKY) rats as controls. SHRs were randomly divided into two groups (n = 10/group), SHR and SHR-T (SHR plus fecal transplantation) and WKY into WKY and WKY-T (WKY plus fecal transplantation). SHR-T received fecal transplantation from WKY, while WKY-T received fecal transplantation from SHR. Blood pressure was measured from the tail artery in conscious rats. 16S rDNA gene amplicon sequencing was used to analyze bacterial composition. Circulating levels of diamine oxidase, D-lactate, FITC-Dextrans, and lipopolysaccharide were determined. Hematoxylin and eosin (H&E) staining was used to observe structural changes in the intestinal mucosa. Immunofluorescence, Western blot, and RT-PCR were utilized to determine changes in the expression of tight junction proteins. Following cross fecal transplantation, blood pressure decreased in SHR and increased in WKY. Significant differences in gut microbial composition were found between hypertensive and normotensive rats, specifically regarding the relative abundance of lactic and butyric acid-producing bacteria. Changes in gut microbiota composition also impacted the intestinal mucosal barrier integrity. Moreover, fecal transplantation affected the expression of tight junction proteins that may impact intestinal mucosal permeability and structural integrity. Blood pressure may be associated with butyric acid-producing intestinal microbiota and its function in regulating the integrity of intestinal mucosal barrier.