文献类型：期刊文献

中文题名：活血定眩胶囊含药血清减轻小鼠脑微血管内皮细胞bEnd.3缺氧损伤

英文题名：Protective effect of Huoxue-Dingxuan capsule-medicated serum on mouse brain microvascular endothelial cell line bEnd.3 with hypoxic injury

作者：刘涛[1];宋敏[1];巩彦龙[1];周灵通[1];董万涛[2];刘建鸿[1]

第一作者：刘涛

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730020

第一机构：甘肃中医药大学

年份：2017

卷号：33

期号：3

起止页码：552

中文期刊名：中国病理生理杂志

外文期刊名：Chinese Journal of Pathophysiology

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(No.81360554);甘肃省自然科学基金资助项目(No.1506RJZA048);甘肃省财政厅基本科研业务课题(No.BH2010-428);甘肃省中药现代制造工程研究院项目(No.YWW-2015049)

语种：中文

中文关键词：活血定眩胶囊;小鼠脑微血管内皮细胞;缺氧;细胞凋亡

外文关键词：Huoxue-Dingxuan capsule;Mouse brain microvascular endothelial cells;Hypoxia;Apoptosis

摘要：目的:研究活血定眩胶囊含药血清对抗缺氧所致小鼠脑微血管内皮细胞bEnd.3损伤的作用。方法:将30只大鼠随机分为空白对照组15只和活血定眩胶囊组15只,采集2组大鼠血清。将bEnd.3细胞分为正常组、缺氧模型组和活血定眩胶囊血清组,给药后,bEnd.3细胞缺氧6 h。显微镜下观察细胞形态,流式细胞术检测细胞凋亡率及细胞周期,按试剂盒方法检测细胞上清液超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果:活血定眩胶囊含药血清可显著对抗缺氧造成的损伤,明显改善bEnd.3细胞的形态,使缺氧所致细胞凋亡数明显减少,有效抑制缺氧诱导的bEnd.3细胞发生G_1/S期阻滞,抑制MDA生成,增强SOD活性。结论:活血定眩胶囊对缺氧所致bEnd.3细胞的损伤具有明显的对抗作用,其机制与增强细胞抗氧化能力、抑制细胞凋亡有关。
AIM：To investigate the protective effect of Huoxue-Dingxuan capsule-medicated serum on mouse brain microvascular endothelial cell line bEnd .3 with hypoxic injury .METHODS：The Wistar rats were randomly divided into control group and Huoxue-Dingxuan capsule group .The serum was collected at the 7th day after drug administration . The bEnd.3 cells were divided into normal group , hypoxia serum model group and Huoxue-Dingxuan capsule serum group . After administration of the medicated serum , bEnd.3 cell were treated with hypoxia for 6 h.The cell morphology was ob-served under microscope , the apoptosis rate and cell cycle were detected by flow cytometry , and the activity of superoxide dismutase （ SOD） and the content of malondialdehyde （ MDA） were detected by ELISA .RESULTS：Compared with other groups, Huoxue-Dingxuan capsule-medicated serum significantly resisted the injury caused by hypoxia , obviously improved the morphology of bEnd .3 cells, significantly reduced the number of apoptotic cells induced by hypoxia , and effectively in-hibited the occurrence of hypoxia-induced G1/S phase arrest in the bEnd.3 cells.At the same time, the medicated serum inhibited MDA production , and increased SOD activity .CONCLUSION： Huoxue-Dingxuan capsule attenuates hypoxia-induced bEnd .3 cell damage by enhancing the antioxidant capacity of cells and inhibiting cell apoptosis .