文献类型：期刊文献

中文题名：针刺对单眼视觉剥夺大鼠视皮层神经元电生理可塑性的调节机制

英文题名：Interventional mechanism of acupuncture on electro-physiological plasticity of visual cortex neurons of monocular visual deprivation rats

作者：刘安国[1];曹朝霞[1];马重兵[1];朱田田[1];张奥[1];李小娟[1];严兴科[1]

第一作者：刘安国

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000

第一机构：甘肃中医药大学针灸推拿学院

年份：2018

卷号：33

期号：5

起止页码：2092

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金项目(No.81660816;No.81260560);甘肃中医药大学中青年创新基金(No.ZQ2015-1)~~

语种：中文

中文关键词：针刺;视觉剥夺;弱视;视皮层;视觉电生理;突触活性区

外文关键词：Acupucture; Visual deprivation; Amblyopia; Visual cortex; Visual electro-physiology; Synaptic activity zone

摘要：目的:利用神经电生理技术研究针刺对单眼视觉剥夺大鼠视皮层神经元电生理可塑性的干预机制。方法:14d龄SPF级幼鼠60只,随机分为空白组,模型组,早、中、末期针刺组。除空白组外,其余各组大鼠通过右眼眼睑缝合制造视觉剥夺大鼠模型,各针刺组选取双侧睛明、攒竹、风池、光明4穴针刺治疗,9d后利用视觉电生理技术及透射电镜对大鼠视皮层神经元形态结构和神经电活动水平进行检测,观察各组大鼠视皮层神经元P-VEP波幅、潜时及电信号活动特点、神经元数目、突触活动区长度等变化。结果:正常组大鼠视皮层神经元放电波形呈规则双向波,动作电位分期明显。而模型组神经元放电波型改变,活动神经元数目明显减少,动作电位分期不明显,P1波峰潜时明显延长(P<0.01),振幅明显缩短(P<0.01),神经元形态结构损害,突触活动区长度缩短(P<0.01),细胞变性及胞核溶解、碎裂。各针刺组治疗后,视皮层神经元电生理活动明显增高,活动神经元数目增加,P1波峰潜时不同程度缩短(P<0.01),P1波振幅明显增高(P<0.05,P<0.01),神经元形态结构向正常水平恢复,突触活性区长度增加(P<0.01)。结论:敏感期内大鼠单眼剥夺后视觉存在明显的功能和结构损伤,针刺能显著改善视觉17区神经元时空特性损害和神经编码异常改变,促进神经元形态结构的恢复,并且疗效与治疗时机密切相关,这为针刺有效干预视觉剥夺效应的神经电生理机制提供了依据。
Objective： To explore the interventional mechanism of acupuncture on electro-physiological plasticity of visual cortex neurons of monocular visual deprivation rats, by using the technique of neurological electrophysiology. Methods： Sixty SPF rats at 14 days age were divided into normal group, model group, early acupuncture group（EAG）, middle acupuncture group（MAG） and advanced acupuncture group（AAG）. The rats model of monocular visual deprivation was established by operating a right eyes tarsorrhaphy. The bilateral acupoints of Jingming（B1）, Cuanzu（B2）, Fengchi（G20） and Guangming（G37） were acupunctured in three acupuncture groups. Nine days after acupuncture treatment, morphological structure of cortical neurons in rats and the nerve electrical activity level were detected by techniques of visual electrophysiology and transmission electron microscope. A series of data in visual cortex were detected, such as amplitude and latency of pattern visual evoked potential（P-VEP）, characteristics of electric signal activity, neurons number and length of synaptic activity zone. Results： In normal group, the neuroelectric waveform of visual cortex was regular and bidirectional, and action potential stage was significant. In model group, the neuroelectric waveform changed, the number of active neurons decreased significantly, action potential stage was not significant, the latency of wave crest of P1 extended significantly（P〈0.01）, amplitude reduced significantly（P〈0.01）, the morphological structure of visual cortex neurons was destroyed, the length of synaptic activity zone shortened（P〈0.05）, cell degeneration, nucleus dissolved and fragmentation appeared. After treatment of each acupunctures groups, the electrophysiological activity of visual cortex neurons increased obviously, the number of active neurons increased, the latency of wave crest of P1 shortened in different degrees（P〈0.01）, the amplitude of P1 increased significantly（P〈0.05, P〈0.01）. the morphological structure of visual cortex neurons returned to normal, the length of synaptic activity zone increased significantly（P〈0.01）. Conclusion： There is obvious damage to the function and structure in visual deprivation rats at the pathologic damage. Acupuncture can significantly improve the spatial and temporal characteristics of neurons in visual 17 areas and the abnormal changes of neural coding, promote the recovery of morphological structure of neurons, and the curative effect is closely related to the time of treatment, which provides a basis for effective intervention of acupuncture effect of visual deprivation neurophysiological mechanism.