文献类型：期刊文献

中文题名：高效液相色谱法同时测定葛根芩连汤煮散及汤剂中6种指标性成分的含量

英文题名：Determination of six index components in Gegen Qinlian Tang(葛根芩连汤)powder for boiling and its decoction by high performance liquid chromatography

作者：窦霞[1,2];杨新荣[3];李硕[4];李倩[4];陈燕[4];钱倩[5];靳子明[1,2]

第一作者：窦霞

机构：[1]甘肃中医药大学附属医院制剂中心,甘肃兰州730020;[2]杨锡仓全国名老中医药专家工作室,甘肃兰州730020;[3]甘肃中医药大学附属医院药学部,甘肃兰州730020;[4]甘肃中医药大学药学院,甘肃兰州730101;[5]甘肃省人民医院药剂科,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2023

卷号：40

期号：6

起止页码：34

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：甘肃省中医药防治重大疾病科研项目(2018ZD04);甘肃省自然科学基金项目(21JR7RA574);甘肃省中医药科研课题(GZKP-2022-23);兰州市城关区科技计划项目(2021-2-9);甘肃省科技计划项目(21JR1RA003);兰州市人才创新创业项目(2021-RC-704);甘肃省人民医院院内科研基金项目(20GSSY4-27)。

语种：中文

中文关键词：葛根芩连汤;指标成分;煮散;高效液相色谱;含量测定

外文关键词：Gegen Qinlian Tang(葛根芩连汤);index components;powder for boiling;high performance liquid chromatography;content determination

摘要：目的测定葛根芩连汤煮散与汤剂中6种指标性成分的含量。方法采用高效液相色谱(HPLC)法测定葛根芩连汤煮散和汤剂中葛根素、大豆苷、黄连碱、黄芩苷、盐酸小檗碱和汉黄芩苷的含量,色谱柱为Phenomenex Gemini C18(250.0 mm×4.6 mm,5.0μm),流动相为乙腈(A)-0.1%甲酸(B)溶液,梯度洗脱,流速1.0 mL/min,检测波长254 nm,柱温30℃,进样量10μL。结果葛根素、大豆苷、黄连碱、黄芩苷、盐酸小檗碱和汉黄芩苷在各自的线性范围内线性关系良好,精密度、重复性、稳定性均符合试验要求,平均加样回收率分别为99.03%,97.98%,98.77%,99.03%,99.02%,98.71%,RSD分别为0.13%,0.90%,0.94%,0.19%,0.24%,0.28%,且煮散中各指标性成分含量均高于汤剂。结论本研究建立的含量测定方法简便、稳定、可行,可为煮散的质量控制、制备工艺及质量标准的制定提供参考。
Objective To determine the content of six index components in Gegen Qinlian Tang(葛根芩连汤)powder for boiling and its decoction.Methods High performance liquid chromatography(HPLC)was used to determine the content of puerarin,daidzein,coptisine,baicalin,berberine hydrochloride,and wogonoside in Gegen Qinlian Tang powder and the decoction.The chromatographic column was Phenomenex Gemini C18(250.0 mm×4.6 mm,5.0μm).The mobile phase is acetonitrile(A)-0.1%formic acid(B)solution,gradient elution,volume flow rate of 1.0 mL/min,detection wavelength of 254 nm,column temperature of 30℃,and injection volume of 10μL.Results The puerarin,daidzein,coptisine,baicalin,berberine hydrochloride,and wogonoside had good linear relationships within their respective linear ranges,and their precision,repeatability,and stability all met the experimental requirements.The average sample recovery rates were 99.03%,97.98%,98.77%,99.03%,99.02%,98.71%,RSD were 0.13%,0.90%,0.94%,0.19%,0.24%,and 0.28%,respectively.The content of each index component in the decoction was higher than that in the decoction.Conclusion The content determination method established in this study is simple,stable,and feasible,and it provides reference for the quality control,preparation process,and quality standard formulation of powder for boiling.