文献类型：期刊文献

中文题名：黄芪多糖对重离子辐射BMSCs防护作用及与NF-κB相关机制研究

英文题名：Protective effect of Astragalus Polysaccharide on heavy ionizing radiation on BMSCs and its mechanism related with NF-κB

作者：张利英[1];王磊[1];张丽昕[1];张苡铭[1];许小敏[1];丁楠[2];华君瑞[2];刘永琦[1,3]

第一作者：张利英

机构：[1]甘肃中医药大学,甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]甘肃省空间辐射生物学重点实验室,中国科学院近代物理研究所,兰州730000;[3]敦煌医学与转化省部共建教育部重点实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2018

卷号：33

期号：12

起止页码：5576

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金项目(No.81473457),甘肃中医药大学中青年项目(No.ZQ2014-11).

语种：中文

中文关键词：重离子;骨髓间充质干细胞;DNA损伤;NF-κB;黄芪多糖

外文关键词：Heavy Ionizing;Bone marrow mesenchymal stem cells;DNA damage;NF-κB;Astragalus Polysaccharide

摘要：目的:研究黄芪多糖(APS)对重离子辐照人骨髓间充质干细胞(BMSCs)促增殖作用、维持其基因组不稳定性以及与NF-κB信号通路相关机制。方法:体外培养人BMSCs,随机分为Ctrl组、APS组、IR组、APS+IR组。Ctrl组为常规BMSCs,APS组用50μg/mL APS干预BMSCs,IR组用2Gy 12C6+辐照BMSCs,APS+IR组用50μg/mL APS干预受辐照BMSCs。CCK-8法和克隆形成实验测各组细胞增殖能力;细胞松弛素法测各组BMSCs的微核率;免疫荧光测53BP1免疫荧光簇集焦点;WesternBlot检测P65、p-P65、COX-2蛋白表达。结果:与Ctrl组比较,12C6+辐射后BMSCs增殖水平降低,克隆形成数减少(P<0.05);微核率和免疫荧光焦点显著增多(P<0.01);P65、p-P65、COX-2等相关蛋白上调(P<0.05,P<0.01)。与IR组比较,APS+IR组BMSCs细胞增殖水平升高,克隆形成数增多(P<0.05);微核率和免疫荧光焦点显著减少(P<0.01,P<0.05);P65、p-P65、COX-2蛋白下调(P<0.05)。结论:APS对2Gy 12C6+辐射BMSCs具有促增长作用,可能和下调NF-κB信号通路相关蛋白,维持BMSCs基因组稳定性有关。
Objective:To study the effect of Astragalus Polysaccharide (APS)on the proliferation level and the protective effect on DNA damage of human bone marrow mesenchymal stem cells (BMSCs)and its mechanism related with NF-κB which was induced by heavy Ionizing Radiation.Methods:BMSCs were randomly divided into Control group (Ctrl),Drug group (APS), Radiation group (IR)and Radiation and Drug group (APS+IR).In Ctrl group,BMSCs cultured with normal medium.In APS group,BMSCs was cultured with 50μg/mL APS.In IR group,BMSCs was radiated by 2Gy 12^C^6+ .In APS+IR group,BMSCs was radiated by 2Gy 12^C^6+ and cultured with 50μ g/mL APS.CCK-8 assay and the cell colony formation assay were used to test the proliferation ability of BMSCs.The micronucleus rate was detected by Cytokinesis-block micronucleus assay,the clustered 53BP1 foci was detected by immunofluorescence test.The expression of P65p-P65 and COX-2 was detected by Western Blot.Results: Compared with Ctrl group,the proliferation rate and the colony formation rate of BMSCs decreased in APS+IR group (P<0.05). The cell micronucleus rate and 53BP1 foci level increased significantly (P<0.01).The expression of P65p-P65 and COX-2up-regulated (P<0.05,P<0.01).Compared with IR group,APS promoted the cell proliferation (P<0.05),reduced cell micronucleus rate and the 53BP1 level (P<0.05),and down regulated the expression of P65p-P65 and COX-2(P<0.05).Conclusion:APS can promote the growth of BMSCs irradiated with 2GY 12^C^6+,which may be related to downregulation of NF-κB signaling pathway related proteins and maintenance of genomic stability of BMSCs.