文献类型：期刊文献

中文题名：萆薢分清颗粒对单侧输尿管梗阻肾纤维化大鼠mTOR、α-SMA、Col-Ⅰ蛋白表达影响研究

英文题名：Effect of Bixie Fenqing granules on the expressions of mTOR,α-SMA and Col-Ⅰ protein in rats with renal fibrosis induced by unilateral ureteral obstruction

作者：李根生[1];马鸿斌[2];魏锦慧[2];何彩苹[1];翟书玲[1];马海兰[1]

第一作者：李根生

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730020

第一机构：甘肃中医药大学

年份：2024

卷号：33

期号：2

起止页码：167

中文期刊名：现代中西医结合杂志

外文期刊名：Modern Journal of Integrated Traditional Chinese and Western Medicine

收录：CSTPCD

基金：甘肃省自然科学基金项目(21JR7RA577);甘肃省名中医传承工作室建设项目(马鸿斌)(甘财社[2022]105号)。

语种：中文

中文关键词：单侧完全性输尿管梗阻;肾纤维化;大鼠;萆薢分清颗粒;雷帕霉素靶蛋白;α-平滑肌肌动蛋白;Ⅰ型胶原

外文关键词：unilateral ureteral obstruction;renal fibrosis;rat;Bixie Fenqing granules;mTOR;α-SMA;Col-Ⅰ

摘要：目的 观察萆薢分清颗粒对单侧输尿管梗阻大鼠肾纤维化相关蛋白雷帕霉素靶蛋白(mTOR)、α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原(Col-Ⅰ)的影响,探讨萆薢分清颗粒的肾脏保护作用。方法 取60只SPF级雄性SD大鼠,从中随机取10只作为空白组,其余50只大鼠采用单侧输尿管梗阻术构建肾纤维化模型。将造模成功大鼠随机分为模型组、氯沙坦组及萆薢分清高、中、低剂量组,每组10只,萆薢分清高、中、低剂量组分别给予13.12 mL/(kg·d)、6.56 mL/(kg·d)、3.28 mL/(kg·d)萆薢分清颗粒溶液灌胃,氯沙坦组给予氯沙坦2.62 mL/(kg·d)灌胃,空白组和模型组给予等量生理盐水灌胃,均1次/d,连续灌胃4周。末次灌胃结束后检测尿白蛋白与尿肌酐的比值(ACR)、24 h尿蛋白量及血肌酐(SCr)、血尿素氮(BUN)水平,HE染色、Masson染色观察肾脏病理形态,免疫组化法及Western blot法分别检测大鼠肾脏组织中mTOR、α-SMA、Col-Ⅰ表达情况。结果 与空白组比较,模型组大鼠ACR、24 h尿蛋白量及SCr、BUN水平均明显升高(P均<0.05);肾脏发生明显纤维化病变;肾组织中mTOR、α-SMA、Col-Ⅰ阳性表达平均光密度值和蛋白相对表达量均明显升高(P均<0.05)。与模型组比较,氯沙坦组与萆薢分清高、中剂量组大鼠ACR、24 h尿蛋白量及SCr、BUN水平均明显降低(P均<0.05);肾纤维化病变较轻;肾组织中mTOR、α-SMA、Col-Ⅰ阳性表达平均光密度值和蛋白相对表达量均明显降低(P均<0.05)。结论 萆薢分清颗粒可减轻单侧输尿管梗阻大鼠肾纤维化,保护残存肾功能,机制可能与下调mTOR、α-SMA、Col-Ⅰ表达有关。
Objective It is to observe the effect of Bixie Fenqing granules on renal fibrosis related proteins mammalian target of rapamycin(mTOR),α-smooth muscle actin(α-SMA) and collagen type Ⅰ(Col-Ⅰ) in unilateral ureteral obstruction rats,and to explore the protective effect of this medicine on renal fibrosis.Methods Sixty SPF male SD rats were selected,in which 10 rats were randomly selected as blank group,and the remaining 50 rats were treated with unilateral ureteral obstruction to establish models of renal fibrosis.The successfully modeled rats were randomly divided into model group,losartan group,and high dose,medium dose and low dose groups of Bixie Fenqing granule,with 10 rats in each group.The high dose,medium dose and low dose groups of Bixie Fenqing granule were respectively given Bixie Fenqing granule 13.12 mL/(kg·d),6.56 mL/(kg·d),3.28 mL/(kg·d) by gavage,the losartan group was given losartan 2.62 mL/(kg·d) by gavage,and the blank group and model group were given the same volume of normal saline by gavage,all once daily,continuously treated for 4 weeks.At the end of the last gavage,the albumin/urine creatinine ratio(ACR),24-hour urinary protein quantification(24 h-UPQ) and the levels of serum creatinine(SCr) and blood urea nitrogen(BUN) were detected,the pathological morphology of the kidneys was observed by HE staining and Masson staining,and the expressions of mTOR,α-SMA,and Col-Ⅰ in renal tissues of the rats were detected by immunohistochemistry and Western blot methods.Results Compared with the blank group,the ACR,24 h-UPQ,SCr and BUN of rats in the model group were significantly increased(all P<0.05),the renal fibrosis was obvious,and the mean optical density values and relative protein expression of mTOR,α-SMA,and Col-Ⅰ in renal tissues were significantly increased(all P<0.05).Compared with the model group,the ACR,24 h-UPQ,SCr and BUN of rats in the losartan group,and high dose,medium dose groups of Bixie Fenqing granule were significantly decreased(all P<0.05),the renal fibrosis was improved,and the mean optical density values and relative protein expression of mTOR,α-SMA,and Col-Ⅰ in renal tissues were significantly decreased(all P<0.05).Conclusion Bixie Fenqing granules can attenuate renal fibrosis and protect residual renal function in rats with unilateral ureteral obstruction,and the mechanism may be related to down-regulating the expressions of mTOR,α-SMA,and Col-Ⅰ.