文献类型：期刊文献

中文题名：中药调控骨髓间充质干细胞增殖及成骨分化相关信号通路的研究进展

英文题名：Chinese Medicine Regulates Signaling Pathways Related to Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells:A Review

作者：杨浩东[1];谢兴文[2];李宁[1,2];黄欣[3];尚征亚[1];姚乾宁[1];刘凯[1]

第一作者：杨浩东

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃中医药大学附属医院,兰州730000;[3]湖南中医药大学,长沙410000

第一机构：甘肃中医药大学

年份：2023

卷号：29

期号：23

起止页码：227

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金项目(82174412,82060873);甘肃省自然科学基金项目(20JR10RA326)。

语种：中文

中文关键词：中药;骨髓间充质干细胞;细胞增殖;成骨分化;信号通路

外文关键词：traditional Chinese medicine;bone marrow mesenchymal stem cells;cell proliferation;osteogenic differentiation;signaling pathways

摘要：骨髓间充质干细胞(BMSCs)来源于从骨髓中分离出的干细胞,具有多向分化和自我更新的潜能,在一定的条件干预下,可将其诱导分化为成骨细胞(OB)、软骨细胞、脂肪细胞和成纤维细胞等。BMSCs对维护骨结构稳定和平衡骨代谢过程具有十分重要的作用,促进BMSCs增殖,诱导其向OB分化对临床防治骨质疏松症、骨缺损、骨折愈合等疾病具有重要的意义。由于BMSCs的增殖及成骨分化过程是一个受多基因控制和多条信号传导通路调控的复杂过程,而中药恰好具有多生物活性成分、多靶点和多通路协同的作用优势,能够通过多渠道影响BMSCs的增殖及分化过程,诱导BMSCs增殖,增强与成骨相关基因的转录与表达,从而促进BMSCs向OB分化,达到防治骨质疏松症、骨缺损等多种骨病的目的。文章通过对中药单体及复方干预BMSCs增殖及成骨分化相关文献进行查阅,重点整理中药单体及复方调控分泌型糖蛋白(Wnt)、神经源性位点缺口同源蛋白(Notch)、丝裂原活化蛋白激酶(MAPK)、磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)、骨形态发生蛋白(BMP)/Smad、Janus激酶(JAK)/信号转导及转录激活因子(STAT)、骨保护素(OPG)/核转录因子-κB受体激活蛋白(RANK)/RANK配体(RANKL)等信号通路促进BMSCs增殖及成骨分化的相关研究进行综述,以期为研究和临床应用中药防治骨科疾病提供新的思路。
Bone marrow mesenchymal stem cells(BMSCs)are derived from stem cells isolated from bone marrow and have the potential for multidirectional differentiation and self-renewal.Under certain conditions,BMSCs can be induced to differentiate into osteoblast(OB),chondrocyte,adipocyte,fibroblast,etc.BMSCs play an important role in maintaining the stability of bone structure and balancing bone metabolism.Promoting the proliferation of BMSCs and inducing their differentiation into OB of great significance for the clinical prevention and treatment of osteoporosis,bone defects,fracture healing,and other diseases.Because the proliferation and osteogenic differentiation of BMSCs are complex processes controlled by multiple genes and regulated by multiple signal transduction pathways,traditional Chinese medicine(TCM)happens to have the advantages of multi-bioactive component,multi-target,and multi-pathway synergism,which can affect the proliferation and differentiation of BMSCs through multiple channels and induce the proliferation of BMSCs.The transcription and expression of genes related to osteogenesis can be enhanced to promote the differentiation of BMSCs into OB,so as to achieve the purpose of preventing and treating osteoporosis,bone defects,and other bone diseases.Based on the literature on the intervention of TCM monomers and compounds in the proliferation and osteogenic differentiation of BMSCs,this study reviewed TCM monomers and compounds in promoting the proliferation and osteogenic differentiation of BMSCs by regulating secreted glycoprotein(Wnt),neurogenic locus notch homolog protein(Notch),mitogen-activated protein kinase(MAPK),phosphatidylinositol-3 kinase(PI3K)/protein kinase B(Akt),bone morphogenetic protein(BMP)/Smad,Janus kinase(JAK)/signal transducer and activator of transcription protein(STAT),osteoprotegerin(OPG)/receptor activator of nuclear factor-kappa B(RANK)/RANK ligand(RANKL),and other signaling pathways to provide new ideas for the research and clinical application of Chinese medicine in the prevention and treatment of orthopedic diseases.