文献类型：期刊文献

英文题名：Temperature-dependent growth and hypericin biosynthesis in Hypericum perforatum

作者：Yao, Yuanyuan[1];Kang, Tianlan[2];Jin, Ling[3];Liu, Zihan[4];Zhang, Zhen[1];Xing, Hua[1];Sun, Ping[1];Li, Mengfei[1,4]

第一作者：Yao, Yuanyuan

通信作者：Li, MF[1]

机构：[1]Gansu Agr Univ, Gansu Prov Key Lab Arid Land Crop Sci, Coll Life Sci & Technol, Lanzhou 730070, Gansu, Peoples R China;[2]Inst Ind Crop & Promot, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China;[4]Gansu Herbal Med Planting Co Ltd, Lanzhou 730000, Gansu, Peoples R China

第一机构：Gansu Agr Univ, Gansu Prov Key Lab Arid Land Crop Sci, Coll Life Sci & Technol, Lanzhou 730070, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, Gansu Prov Key Lab Arid Land Crop Sci, Coll Life Sci & Technol, Lanzhou 730070, Gansu, Peoples R China.

年份：2019

卷号：139

起止页码：613

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;Scopus(收录号：2-s2.0-85064636440);WOS:【SCI-EXPANDED(收录号:WOS:000469896200064)】；

基金：This work was financially supported by the fund of Gansu Agricultural University (GSCS-2018-1, GAU-XKJS-2019 and Gaufx-02J04), National Natural Science Foundation of China (81560617), Institute of Agricultural and Animal Husbandry of Gansu Province, China (GNCX-2016-12), and Innovative Groups for Fundamental Research in Gansu Province, China (1606RJIA323).

语种：英文

外文关键词：Hypericum perforatum; Temperature; Growth; Hypericin biosynthesis; Gene expression

摘要：Hypericum peyforatum is a perennial herb that produces the anti-depression metabolite hypericin (Hyp). While several efforts to increase Hyp production have been made, the effects of temperatures on growth and Hyp biosynthesis are still limited. In this study, the growth morphophysiological traits, Hyp biosynthesis and their related genes expression, as well as major bioactive compounds accumulation and antioxidant capacity were assessed by exposing H. perforatum seedlings to three different temperatures (15, 22 and 30 degrees C). The results showed that aerial parts biomass was greater at 15 degrees C with 1.3 and 1.6-fold increase compared to at 22 and 30 degrees C, in large part because of greater increase in chlorophyll content, stem number and leaf area on a per plant basis. Hyp content in the aerial parts was greater 1.9 and 5.6-fold on a per plant basis compared to 22 and 30 degrees C treatments, and the contents of other bioactive compounds (flavonoids and phenolics) as well as antioxidant capacity in the aerial parts, on dry weight and per plant basis, also exhibited significant increases with the temperatures decrease. The mRNA expressions of eight genes (psbA, psbB, psbC, psbD, ycf3, ycf4, ycf5 and matK) related to photosynthesis and two genes (Polyketide synthase, PKS; Phenolic oxidative coupling protein, Hyp-1) involved in Hyp biosynthesis were also up-regulated at 15 degrees C. The findings are useful in guiding cultivation and regulating Hyp biosynthesis in H. perforatum.