文献类型：期刊文献

英文题名：Simultaneous Determination of 10 Components in Bu-Zhong-Yi-Qi Wan by Solid Phase Extraction-High Performance Liquid Chromatography with Diode Array Detection and Evaporative Light Scattering Detection

作者：Hu, Fang[1];Zhu, Ruijuan[1];Liu, Xiaohua[1];Yang, Yinglai[1];Li, Can[1];Feng, Shilan[1];Li, Yingdong[2]

第一作者：Hu, Fang

通信作者：Feng, SL[1]

机构：[1]Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Dept Pharm, Lanzhou 730000, Gansu, Peoples R China

第一机构：Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Pharm, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2015

卷号：53

期号：5

起止页码：736

外文期刊名：JOURNAL OF CHROMATOGRAPHIC SCIENCE

收录：;Scopus(收录号：2-s2.0-84941657957);WOS:【SCI-EXPANDED(收录号:WOS:000360833600013)】；

基金：The study was financially supported by National Science and Technology Supporting Program for the 12th Five-Year Plan (NO. 2011BAI05B02).

语种：英文

摘要：An effective, accurate and reliable method for the simultaneous separation and determination of 10 major components in Chinese medicine Bu-Zhong-Yi-Qi Wan (BZYQW) was developed and validated using solid phase extraction column-high performance liquid chromatography-diode array detection-evaporative light scattering detection (SPE-HPLC-DAD-ELSD). The chromatographic separation was performed on a Spursil (TM) C-18 column (250 mm x 4.6 mm, 5 mm) at 30 degrees C with an acetonitrile-water gradient as mobile phase. The DAD detection wavelength 254 nm was utilized for the quantitative analysis. The drift tube temperature and the carrier gas flow rate of the ELSD detection was set at 110.5 degrees C and 3.1 mL/min. The total run time is 103 min, these determined components peak out within 81 min. Excellent linear behaviors over the investigated concentration ranges were observed with the values of r(2) higher than 0.9990 for all the analytes. The Linear range over hesperidin, senkyunolide I, senkyunolide H, ononin, calycosin, formononetin, ligustilide, butylene phthalide, astragaloside IV, astragaloside I is 4.50-94.50 mu g/mL, 22.75-364.00 mu g/mL, 2.30-45.00 mu g/mL, 11.76-125.14 mu g/mL, 4.62-50.35 mu g/mL, 1.90-28.93 mu g/mL, 1.29-159.00 mu g/mL, 2.90-36.00 mu g/mL, 35.40-192.40 mu g/mL, 41.40-96.60 mu g/mL, respectively. The method was validated by its repeatability [relative standard deviation (RSD) < 3.54%] and intra-day (RSD < 2.11%) and inter-day precision (RSD < 3.45%). The limits of detection and quantification of each component were in the ranges of 0.04-10.24 and 0.1239.22 mu g/mL, respectively. The average recovery yields of the 10 compounds ranged from 95.79 to 101.25%. The validated method was successfully applied to the simultaneous determination of these principal components in 10 commercial samples of BZYQW from different manufacturers.