文献类型：期刊文献

中文题名：不同采收期对甘草中甘草苷、甘草酸及甘草多糖含量的影响

英文题名：Effects of harvest time on the contents of liquirtin, glycyrrhizic acid and polysaccharide in licorice

作者：李越峰[1,2];边甜甜[1,2];司昕雷[1,2];牛江涛[1,2];曹瑞[1,2];何晓军[3]

第一作者：李越峰

机构：[1]甘肃中医药大学;[2]甘肃省中药质量与标准研究重点实验室;[3]甘肃省疾病预防控制中心

第一机构：甘肃中医药大学

年份：2016

卷号：0

期号：5

起止页码：47

中文期刊名：中兽医医药杂志

外文期刊名：Journal of Traditional Chinese Veterinary Medicine

基金：国家自然科学基金资助项目(81460611);教育部科学技术研究重点基金资助项目(212186);甘肃省自然科学基金资助项目(2010:1010RJZA212;2014:145RJZA076);甘肃省财政厅高校基本科研业务费专项基金资金项目(2013-2);甘肃中医学院引进人才科研启动基金资助项目(2010-3)

语种：中文

中文关键词：甘草;甘草苷;甘草酸;甘草多糖;采收期;高效液相色谱

外文关键词：licorice; liquirtin; glycyrrhizic acid; harvest; HPLC

摘要：目的:建立测定甘草中甘草苷、甘草酸及甘草多糖的方法,并比较不同采收时期甘草中甘草苷、甘草酸及甘草多糖的差异。方法:采用HPLC法测定甘草中甘草苷和甘草酸含量,色谱柱为Kromasil C_(18)柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.05%磷酸溶液,梯度洗脱;体积流量为1 m L/min;检测波长为252 nm、276 nm;柱温为28℃;进样量为10μL,并采用紫外吸收光谱法测定甘草中甘草多糖。结果:甘草苷和甘草酸获得良好分离,在0.116 8-1.168 0μg及0.039 3-0.235 0μg与峰面积积分值呈良好线性关系,平均加样回收率分别为98.45%、98.34%,RSD分别为1.52%、1.78%,重现性试验RSD分别为0.35%、0.59%。甘草多糖分离良好,其重现性、稳定性亦良好,回收率较高。结论:本方法操作简便、结果准确、精密度好、分离度良好,为甘草药材的合理应用及其质量控制提供了依据。
Objective： To establish a determination method of liquirtin, glycyrrhizic acid and polysaccharide in licorice, and study the effect of different harvest period on their contents. Methods： HPLC method was applied for quality assessment of licorice with Kromasil C_（18） column（4.6 mm×250 mm, 5μm）, flow-rate of 1.0 m L/min, column temperature of 28 ℃, mobile phase of acetonitrile and 0.05% phosphoric acid, respectively. The content of polysaccharide licorice was determined by ultraviolet absorption spectrometry. Results： The linear ranges of liquirtin and glycyrrhizic acid were 0.116 8-1.168 0 μg and 0.039 3-0.235 0 μg,respectively. The average recoveries of liquirtin and glycyrrhizic acid were 98.45%（RSD 1.52%） and 98.34%（RSD 1.78%）.Licorice polysaccharide could be separated effectively with good repeatability, stability and recovery. Conclusion：Determination of these active components in licorice root with high specificity can be used to control its quality and assure lot to lot consistency.