文献类型：期刊文献

英文题名：A rapid and specific fluorescent probe based on aggregation-induced emission enhancement for mercury ion detection in living systems

作者：An, Yong[4];Li, Bo[4];Yu, Yongzhi[4];Zhou, Yucen[2];Yi, Jianfeng[1];Li, Lepeng[1];Sun, Yongqiang[1];Qiang, Zhengze[3];Liu, Yongqi[2];Wang, Peng[5]

第一作者：An, Yong

通信作者：Liu, YQ[1];Qiang, ZZ[2];Wang, P[3]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Key Lab Mol Med & Chinese Med Prevent &, Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou 730000, Gansu, Peoples R China;[4]Gansu Prov Canc Hosp, Dept Musculoskeletal Tumor, Lanzhou 730000, Gansu, Peoples R China;[5]China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637009, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Key Lab Mol Med & Chinese Med Prevent &, Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou 730000, Gansu, Peoples R China;[3]corresponding author), China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637009, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：465

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20235215286703);Scopus(收录号：2-s2.0-85180579755);WOS:【SCI-EXPANDED(收录号:WOS:001146042500001)】；

基金：This work were supported by the National Natural Science Foundation of China (No. 82303568 and No. 22206154), Gansu Provincial Natural Science Foundation (No. 23JRRA1216, No. 21JR11RA140 and No. 21JR7RA560), Gansu Provincial University Innovation Foundation (No. 2023QB-085, No. 2021B-161 and No. 2022A-063), the Introduced Talent Research Start-up Fund (No. 2023-YJRC-16), Scientific Research and Innovation Fund (No. 2021-KCYB-07) of Gansu University of Chinese Medicine.

语种：英文

外文关键词：Aggregation-induced emission; Mercury ions; Peptide; Oxidative stress; Biomimetic probe

摘要：Mercury is a harmful heavy metal that seriously threatens the environment and organisms. In this study, we combined the aggregation-induced emission mechanism and the advantages of peptides to design a novel tetraphenylene (TPE)-based peptide fluorescent probe, TPE-Cys-Pro-Gly-His (TPE-CPGH), in which the sulfhydryl group of Cys in the peptide chain and the imidazolium nitrogen provided by His were used to mimic the Hg2+ binding site of metalloproteins. The beta-fold formed by Pro-Gly was used to promote the spatial coordination of the probe with Hg2+ and the formation of the coordination complex aggregates, these changes led to the "turn on" response to Hg2+. The detection of Hg2+ by TPE-CPGH not only showed high specificity and sensitivity (LOD=46.2 nM), but also had the advantages of fast response and applicability for detection over a wide pH range. Additionally, TPE-CPGH effectively detected Hg2+ in environmental samples, living cells and organisms due to its low cytotoxicity, high water solubility and cell membrane permeability. More interestingly, TPE-CPGH was also mitigated Hg2+ exposure-induced oxidative stress toxicity in vitro and in vivo.