文献类型：期刊文献

中文题名：湿生扁蕾总口山酮对NCM460细胞间质转化的影响

英文题名：Effect of Xanthones from Gentianopsispaludosa on Mesenchymal Transformation of NCM460 Cells

作者：柳娜[1];刘越敏[1];薄双琴[1];陈晖[1];张尚智[1];景明[1]

第一作者：柳娜

机构：[1]甘肃中医药大学,兰州730000

第一机构：甘肃中医药大学

年份：2022

卷号：44

期号：9

起止页码：1735

中文期刊名：中国细胞生物学学报

外文期刊名：Chinese Journal of Cell Biology

收录：CSTPCD;;CSCD:【CSCD_E2021_2022】；

基金：国家自然科学基金(批准号:81860788、82160854);兰州市人才创新创业项目(批准号:2019-RC-1017)资助的课题。

语种：中文

中文关键词：湿生扁蕾总口山酮;溃疡性结肠炎;结肠上皮–间质转化

外文关键词：Xanthones from Gentianopsispaludosa;ulcerative colitis;colon epithelial-mesenchymal transformation

摘要：该研究探究了湿生扁蕾总口山酮对人结肠上皮细胞(normal colon mucosa cell,NCM460)间质转化的影响。采用2.5 μg/mL脂多糖(LPS)诱导NCM460细胞14天建立间质转化模型,分别以湿生扁蕾总口山酮(GPX)及吡菲尼酮(PFD)进行干预,以免疫荧光(IF)和蛋白免疫印迹技术(WB)以及实时荧光定量转录聚合酶链反应(RT-qPCR)分别检测E-cadherin和Vimentin蛋白和mRNA的表达。结果显示,与正常对照组相比,模型对照组细胞中上皮细胞标志物E-cadherin蛋白和mRNA表达明显下调(P<0.01),间质细胞标志物Vimentin蛋白和mRNA表达明显上调(P<0.01),与模型组相比,E-cadherin的荧光强度与GPX剂量呈正相关,Vimentin荧光强度呈剂量依赖性降低。GPX中、高剂量组E-cadherin蛋白和mRNA表达上调,Vimentin蛋白和mRNA表达下调(P<0.01),以高剂量最为明显。研究表明,湿生扁蕾总口山酮能够有效抑制结肠上皮–间质转化,这为其进一步研究开发提供理论依据。
This study investigated the effects of Xanthones from Gentianopsispaludosa on human NCM460 (normal colon mucosa cell) interstitial conversion.NCM460 cells were induced with 2.5 μg/mL LPS (lipopolysaccharide) for 14 d to establish interstitial transformation model,and were treated with GPX and PFD respectively.The expression of E-cadherin and Vimentin protein and mRNA were detected by IF (immunofluorescence),WB (Western blot) and RT-qPCR (real-time fluorescence quantitative transcription polymerase chain reaction),respectively.The results showed that compared with normal control group,the epithelial cell marker E-cadherin protein and mRNA in model control group were significantly down-regulated (P<0.01),interstitial cell marker Vimentin protein and mRNA were significantly up-regulated (P<0.01).Compared with the model group,the fluorescence intensity of E-cadherin was positively correlated with the dose of GPX,while the fluorescence intensity of Vimentin was decreased in a dose-dependent manner.The expression of E-cadherin protein and mRNA was up-regulated and that of Vimentin protein and mRNA was down-regulated in GPX medium and high dose groups (P<0.01),most obvious in high dose.The study showed that Xanthones from Gentianopsispaludosa could effectively inhibit colon EMT (epithelial-mesenchymal transformation),which provided theoretical basis for further research and development.