文献类型：期刊文献

英文题名：The pharmacodynamic active components study of Tibetan medicine Gentianopsis paludosa on ulcerative colitis fibrosis

作者：Lu, Nian-hua[1];Zhao, Hui-qiao[1];Jing, Ming[1];Liu, Xiong[1];Ren, Chun-zhen[1];Liu, Xue-feng[1];Liu, Juan-juan[1];Zhang, Yan-xia[1]

第一作者：Lu, Nian-hua

通信作者：Jing, M[1]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2017

卷号：46

起止页码：163

外文期刊名：INTERNATIONAL IMMUNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85014819089);WOS:【SCI-EXPANDED(收录号:WOS:000399259100022)】；

基金：The authors are grateful to Prof. Chen Yuan of Gansu Agricultural University for identifying the plant, and Gannan Tibetan Autonomous science and Technology Bureau of Gansu Province. This research was partially supported by the National Natural Science Foundation of China (no. 81360522; no. 81560717) and Animal Experimental Center of Gansu University of Chinese Medicine (no. SCXK < GAN > 20160002).

语种：英文

外文关键词：Tibetan medicine; Gentianopsis paludosa; Ulcerative colitis fibrosis; Active component

摘要：The current study focused on the pharmacodynamic activity components of Gentianopsis paludosa against ulcerative colitis (UC) fibrosis including symptoms of intestinal diarrhea and inflammatory. Trinitro-benzene-sulfonic acid induced UC model rats were gavaged with gradient polarity extracts respectively from-ethanol-extract of Gentianopsis paludosa. Masson staining and qRT-PCR methods were respectively used to assess the degree of UC fibrosis and detect the mRNA expressions of collagen I, collagen III, a-smooth muscle actin (alpha-SIVIA) and E-cadherin in colon tissue. Separated by silica gel column chromatography, further screening was conducted until active components appeared. Infrared, nuclear magnetic resonance, mass spectroscopy and ultraviolet methods were applied to confirm active components' structures. The results indicated that the expression of collagen I, collagen III and alpha-SMA mRNA in the colon tissues of acetidin group rats was obviously depressed compared with control groups while E-cadherin displayed just opposite. Dyed in blue indicating UC fibrosis degree, the area of acetidin group was less than that other experimental groups. Four components: (1,8-Dihydroxy-3,7-Dimethoxyxanthones, 1-hydroxy-3,7,8-Trimethoxyxanthones, 1,7-Dihydroxy-3,8-Dimethoxyxanthones and 1-hydroxy-3,7-Dimethoxyxanthones), were obtained from acetidin group and all of which have a significant equivalence to Gentianopsis paludosa on the therapeutic effect of UC fibrosis. Our findings revealed the activity components for clinical application history of Gentianopsis paludosa and provided a preliminary foundation for further new drug research and exploitation. (C) 2017 Elsevier B.V. All rights reserved.