文献类型：期刊文献

中文题名：红芪多糖对GK糖尿病胃轻瘫大鼠胃窦组织MYPT1/p-MYPT1蛋白表达的影响

英文题名：Effect of Hedysari polysaccharide on Expression of Protein in MYPT1/p-MYPT1 from Gastric Antrum in GK rat with Diabetic Gastroparesis

作者：万生芳[1];李雅琪[2];舒畅[1];王晓丽[1];魏昭晖[1];张磊[1];李永玉[1];吴红彦[1]

第一作者：万生芳

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]中国药科大学,江苏南京211198

第一机构：甘肃中医药大学

年份：2019

卷号：30

期号：1

起止页码：33

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金(81560718);全国第四批中医(临床;基础)优秀人才培养项目(9986010603);甘肃省中医药管理局科研课题项目(GZK-2017-3);甘肃省普通本科高等学校基本科研业务费项目(2305136310)

语种：中文

中文关键词：糖尿病胃轻瘫;红芪多糖;GK大鼠;胃激素;RhoA/RocK信号通路

外文关键词：Diabetic gastroparesis;Hedysari polysaccharide;GK(Goto-Kakizaki)rat;Gastric hormone;RhoA/RocK signaling pathway

摘要：目的探讨红芪多糖(HPS)改善GK糖尿病胃轻瘫(DGP)大鼠胃动力的作用机制。方法 12只Wistar大鼠作为正常组,68只GK大鼠高糖高脂饲料不规则喂养8周制备DGP模型,60只成模大鼠随机分为模型组、阳性药组(莫沙必利)、红芪多糖低、中、高剂量组等5组(每组12只)。除正常组和模型组外,其他各组大鼠灌胃干预12周。于干预前和干预后第4、8、12周末检测血糖;实验结束后检测胃排空率(GER);ELISA法检测胃泌素(GAS)、胃动素(MTL)、P物质(SP)含量;分别采用免疫组化法和蛋白印迹(WB)法检测胃窦组织RhoA、MYPT1、p-MYPT1蛋白表达情况。结果与正常组比较,模型组大鼠血糖显著升高(P<0.01),胃排空率显著下降,胃激素含量显著降低,胃窦组织RhoA、MYPT1、p-MYPT1蛋白表达显著降低(P<0.01);经干预治疗后,与模型组比较,阳性药组和HPS各组血糖显著降低(P<0.01),胃排空率、胃激素含量、胃窦组织RhoA、MYPT1、p-MYPT1蛋白表达量显著增高(P<0.05或P<0.01)。结论 HPS能够降低GK糖尿病胃轻瘫大鼠血糖,促进胃排空,增加胃泌素、胃动素、P物质等胃激素的含量,这可能与其调节胃窦组织RhoA/RocK信号通路有关。
Objective To explore mechanism of promoting gastric motility of Hedysari Polysaccharide(HPS)on GK rat with diabetic gastroparesis.Methods 12 Wistar rats were used as the normal group,68 GK rats were induced to be DGP model by irregular feeding for 8 weeks,then the model rats were randomly divided into 5 groups:model group,positive drug group(mosapride),low-dose,middle-dose,high-dose HPS(HPS-L,HPS-M,HPS-H)group,each group had 12 rats.All rats except normal and model group were given continuous intragastric administration for 12 weeks.Blood-glucose(GLU)level was detected at the baseline before intervention and at the end of 4,8,12 weeks after intervention separately.Gastric emptying rate(GER)was detected.The concentration of plasma motilin(MTL),serum gastrin(GAS),substance P(SP)were detected by elisa method.The expression of protein in RhoA,MYPT1,p-MYPT1 were detected by immunohistochemical and western blot(WB)method respectively.Results Compared with normal group,blood glucose level increased significantly(P<0.01),gastric emptying rate,the concentration of gastric hormone and protein expression level of RhoA,MYPT1,p-MYPT1 decreased significantly in model group(P<0.01).12 weeks later,compared with model group,blood glucose level decreased significantly(P<0.01),gastric emptying rate,the concentration of gastric hormone,protein expression level of RhoA,MYPT1,p-MYPT1 of gastric antrum increased significantly in positive drug group and HPS-H group(P<0.05 or P<0.01).Conclusion HPS may decrease level of blood-glucose,promote gastric emptying,increase concentration of MTL,GAS and SP.All of these may be relatived to regulating RhoA/RocK signaling pathway.