文献类型：期刊文献

中文题名：干旱胁迫对党参苯丙素及倍半萜和三萜类成分合成相关基因的调控

英文题名：Genetic adaptation under drought stress for the synthesis of phenylpropanoid,sesquiterpenoids and triterpenoids in Codonopsis pilosula

作者：孙晓琛[1,2];原静静[1,2];栗锦鹏[1,2];陈彦宏[1,2];张博洋[1,2];王惠珍[1,2]

第一作者：孙晓琛

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]西北中藏药省部共建协同创新中心,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2022

卷号：44

期号：12

起止页码：3902

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金(81760683,81960683);国家现代农业产业技术体系资助项目(2022);甘肃省教育厅“双一流”重点项目(GSSYLXM-05)。

语种：中文

中文关键词：党参;干旱胁迫;苯丙素类;倍半萜;三萜类;关键基因

外文关键词：Codonopsis pilosula(Franch.)Nannf.;drought stress;penylpropanoids;sesquiterpenes;triterpenes;key genes

摘要：目的对不同干旱胁迫程度下处于生长旺盛期党参的根、茎、叶组织进行转录组测序分析,探索干旱胁迫对党参根、茎、叶中苯丙素类、倍半萜和三萜类次生代谢产物合成相关基因的调控。方法通过盆栽控水进行党参干旱胁迫处理,设置CK(正常水分)、LD(轻度干旱)、MD(中度干旱)、SD(重度干旱),分别为田间最大持水量的90%~85%、70%~65%、55%~50%、40%~35%。利用Illumina HiSeq 2500高通量测序技术对生长旺盛期党参根、茎、叶组织进行测序,获得党参差异基因后进行生物信息学分析。结果筛选出干旱胁迫下党参根、茎、叶组织中苯丙素生物合成的关键基因10个,其中叶10个,茎7个,根7个,且轻度干旱胁迫下茎中关键基因上调高表达;筛选出干旱胁迫下参与党参倍半萜和三萜类生物合成差异表达关键酶基因13个,其中叶13个,茎1个,根4个,且轻度干旱下叶中关键基因上调高表达。结论干旱胁迫能在一定程度上促进党参中苯丙素类、倍半萜和三萜类生物合成关键基因的表达,不同组织和不同干旱程度的基因上调/下调程度有差异,为通过分子手段调控党参次级代谢产物合成奠定基础。
AIM To analyze the transcriptome sequencing of Codonopsis pilosula(Franch.)Nannf.in the root,stem and leaf tissues at the peak growth stage when exposed to different levels of drought stress,and explore the genetic adaptation under drought stress for the biosynthesis of phenylpropanoid,sesquiterpenoid and triterpenoid in the roots,stems and leaves of C.pilosula.METHODS Soil water content of the container seedling was controlled to a specified range by four different drought treatments,of CK(normal water),LD(mild drought),MD(moderate drought)and SD(severe drought),which were 90%-85%,70%-65%,55%-50%and 40%-35%of the maximum water holding capacity in the field,respectively.Illumina HiSeq 2500 high-throughput sequencing technology was used to sequencing the root,stem and leaf tissues of C.pilosula during the flourishing period,and further bioinformatic analysis was performed upon the obtained differential genes.RESULTS Among the proven ten key enzyme genes involving in phenylpropanoid biosynthesis in root,stems and leaf tissues of C.pilosula under drought stress,ten in leaf,seven in stem and seven in root were found.And mild drought stress found upregulation and high expression of the key genes in stem..Thirteen differentially expressed key enzyme genes in sesquiterpenoid and triterpenoid biosynthesis of C.codonopsis under drought stress were identified,including thirteen in leaf,one in stem and four in the root.And mild drought stress found upregulation and high expression of the key genes in the leaves.CONCLUSION Drought stress promotes somewhat the key gene expression of phenylpropanoids,sesquiterpenes and triterpenes biosynthesis of C.pilosula,with variations in extent of gene up-regulation and down-regulation in different tissues due to different drought exposure,highlighting the molecular regulation of the synthesis of secondary metabolites of C.pilosula.