文献类型：期刊文献

中文题名：桔梗过氧化物酶Ⅲ基因家族的鉴定及辐射后表达分析

英文题名：Identification of Peroxidases ClassⅢGene Family and Its Expression Analysis After Irradiation in Platycodon grandiflorus

作者：刘霄[1,2];杜艳[1,2];李雪虎[1,2];晋玲[3];马晓辉[3];王富胜[4];周利斌[1,2,5]

第一作者：刘霄

机构：[1]中国科学院近代物理研究所生物物理室,甘肃兰州730000;[2]中国科学院大学,北京100049;[3]甘肃中医药大学药学院,甘肃兰州730000;[4]定西市农业科学院,甘肃定西743000;[5]白银市科近重离子束生物产业创新研究院,甘肃白银730900

第一机构：中国科学院近代物理研究所生物物理室,甘肃兰州730000

年份：2023

卷号：37

期号：6

起止页码：1089

中文期刊名：核农学报

外文期刊名：Journal of Nuclear Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：核能开发科研项目“核辐射作物品种改良与害虫防控”;中国科学院青年创新促进会(Y201974);道地药材生态种植及质量保障项目(国中医药科技[2020]153号);定西市科技计划(DX2021AN10)。

语种：中文

中文关键词：桔梗;过氧化物酶Ⅲ;基因家族;电离辐射;重离子束

外文关键词：Platycodon grandiflorus;Peroxidases classⅢ;gene family;ionizing radiation;heavy ion beam 1098

摘要：为探究过氧化物酶Ⅲ(peroxidase classⅢ,PERs)基因家族在药用植物响应电离辐射中的作用,本试验研究了中药材桔梗的PERs(PgPERs)家族成员特性,及桔梗幼苗经高能重离子束和X射线辐射处理后PgPERs基因的表达量变化。通过序列比对、结构域鉴定和蛋白保守基序分析,共获得53个PgPERs,其蛋白的序列长度多数为258~621个氨基酸,碱性蛋白占多数。启动子区调控元件分析结果说明PgPERs存在多个与生长发育、植物激素和逆境胁迫相关的顺式作用元件。进化分析结果显示,PgPERs基因家族主要分为5个簇,与拟南芥过氧化物酶Ⅲ(AtPERs)有29对共线性关系。高能重离子束辐射桔梗幼苗后检测到3个PgPERs基因表达量上调,X射线辐射处理后有8个PgPERs基因差异表达。这些差异基因编码的蛋白质二级结构以α-螺旋和无规则卷曲为主,三级结构的相似度高,并含有跨膜螺旋。以上结果表明,不同类型的电离辐射处理桔梗幼苗后,其PgPERs响应机制并不相同。本研究结果为探究电离辐射影响桔梗生长发育和辐射响应过程提供了新的视角。
In order to explore the role of peroxidases classⅢ(PERs)in the response of medicinal plants to ionizing radiation,this study investigated the characteristics of PgPERs family in Platycodon grandiflorus and the expression regulation of PgPERs in seedlings after high energy heavy ion beam and X-ray radiation.Totally 53 PgPERs were obtained by sequence alignment,domain identification and protein conserved motif analysis.The length of protein sequences are mainly between 258 and 621 amino acids,and the majority are alkaline protein.The analysis of promoter regulatory elements showed that PgPERs have many cis-acting elements related to growth and development,plant hormone and stress response.According to the evolutionary analysis,the PgPERs are mainly divided into five clusters,29 PgPERs showed collinearity with AtPERs in Arabidopsis thaliana.The expression of three PgPERs genes are up-regulated after high energy heavy ion beam irradiation,while eight PgPERs genes are differentially expressed after X-ray irradiation.The secondary structures of the proteins encoded by the differential genes are dominantlyα-helix and random coil.The tertiary structures are highly similar and contain transmembrane helices.The results demonstrated that PgPERs have different response mechanisms to different ionizing radiations.This study provided a new perspective for exploring the effects of ionizing radiation on the growth and development and radiation respone process of platycodon grandiflorus.