文献类型：期刊文献

中文题名：葛根素对糖尿病大鼠肾脏糖基化终产物和转化生长因子-β_1表达的影响

英文题名：Effects of Puerarin on Renal Advanced Glycosylation End Products and Expression of TGF-β_1 in Diabetic Rats

作者：李长天[1];陈雁飞[2]

第一作者：李长天

机构：[1]甘肃中医学院,甘肃兰州730000;[2]兰州大学第一医院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2006

卷号：13

期号：3

起止页码：36

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：甘肃中医药管理局资助项目(2003-GZK-48)

语种：中文

中文关键词：葛根素;糖尿病肾病;糖基化终产物;转化生长因子-β1

外文关键词：puerarin; diabetic nephropathy; AGEs： TGF-β1

摘要：目的研究葛根素对糖尿病大鼠肾脏糖基化终产物(AGEs)和转化生长因子-β1(TGF-β1)表达及肾脏病理形态学的影响。方法大鼠随机分为正常对照组(CN组)、糖尿病模型组(DM组)和葛根素治疗组(DP组)。葛根素处理10周后,测定血糖、尿白蛋白、肾小球基底膜厚度(GBMT)和肾脏肥大指数,以光镜和电镜观察肾脏病理改变。用荧光显微镜曝光时间法测定肾组织AGEs含量,用免疫组化法检测肾组织TGF-β1的表达。结果糖尿病大鼠尿白蛋白、肾脏肥大指数、GBMT均显著高于正常对照组(P<0.01),葛根素处理后上述指标均明显下降(P<0.01)。葛根素治疗组的肾组织AGEs荧光曝光时间较糖尿病模型组延长(P<0.05),肾组织TGF-β1的表达阳性率明显低于糖尿病模型组(P<0.01),并且肾脏病理改变也有所改善。结论糖尿病大鼠肾组织中AGEs含量及TGF-β1的表达均明显增加。葛根素可以减少其肾组织AGEs含量,下调TGF-β1的表达,改善糖尿病大鼠早期肾脏病理改变。
Objective To investigate the effects of puerarin on renal advanced glycosylation end products （AGEs）, expression of transforming growth factor-β1 （TGF-β1） and pathomorphism in diabetic rats. Methods Rats were randomly divided into following groups： normal control rats （Group CN）, diabetic rats （Group DM） and diabetic rats treated with puerarin （Group DP）. After Puerarin was given to STZ-induced diabetic rats for 10 weeks, blood glucose, urine protein, glomerular basement membranee thickness （GBMT） and profile of kidney hypertrophy in diabetic rats were measured and analysed. The renal pathologic changes were observed by light microscopy and electron microscopy. Renal AGEs content was determined by expose time of fluorescence microscopy. The expression of TGF-β1 was examined by immunohistochemical methods. Results Urine protein, GBMT and profile of kidney hypertrophy were significantly increased in diabetic rats as compared with control animals （P〈0.01）, but those targets in Group DP were decreased （P〈0.01）. The fluorescence expose time of renal AGEs in group DP was longer than that in group DM （P〈0.05）. The expression of TGF-β1 in group DP was dramatically lower than that in group DM （P〈0.01）, meanwhile, renal pathologic changes were also improved in group DP. Conclusion Renal AGEs contents and expression of TGF-β1 are so significantly increased in diabetic rats. Administration of puerarin to diabetic rats can ameliorate diabetic nephropathy. Such beneficial effect can be related to reduction of AGEs centents and inhibition of the expression of TGF-β1 in the kidney by puerarin.