文献类型：期刊文献

中文题名：久泻灵颗粒对脾肾阳虚型溃疡性结肠炎大鼠相关细胞因子的影响

英文题名：Effects of Jiuxieling Granules on IL-1, IL-6, TNF-α, and IFN-γ in Rats with Spleen Kidney Yang Deficiency Ulcerative Colitis

作者：许雅清[1];李海龙[1,2];邱家权[1];贾育新[1];程小丽[1];明海霞[1];梁永林[1];吴玉泓[1]

第一作者：许雅清

机构：[1]甘肃中医学院,甘肃兰州730000;[2]甘肃中医学院甘肃省中药新产品创制工程实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2015

卷号：22

期号：4

起止页码：59

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：国家自然科学基金(81260520)

语种：中文

中文关键词：久泻灵颗粒;溃疡性结肠炎;脾肾阳虚;白细胞介素-1;白细胞介素-6;肿瘤坏死因子-α;干扰素-γ;大鼠

外文关键词：Jiuxieling Granules; ulcerative colitis; spleen and kidney yang deficiency; IL-1; IL-6; TNF-α; IFN-γ; rats

摘要：目的探讨久泻灵颗粒治疗脾肾阳虚型溃疡性结肠炎的作用机制。方法采用灌服大黄水煎液+肌肉注射氢化可的松并结合2,4,6-三硝基苯磺酸+乙醇灌肠建立动物模型。将90只大鼠随机分为空白组、模型组、柳氮磺吡啶组和久泻灵颗粒7、14、21 d组,各治疗组给予相应药物干预。采用酶联免疫法检测各组大鼠血清及结肠组织中白细胞介素(IL)-1、IL-6、肿瘤坏死因子-α(TNF-α)及干扰素-γ(IFN-γ)含量。结果与空白组比较,模型组大鼠血清及结肠组织中IL-1、IL-6、TNF-α及IFN-γ含量均升高(P<0.05);与模型组比较,各治疗组大鼠血清及结肠组织中IL-1、IL-6、TNF-α及IFN-γ含量均降低(P<0.05),其中久泻灵颗粒21 d组作用最明显(P<0.05)。结论久泻灵颗粒通过调节脾肾阳虚溃疡性结肠炎模型大鼠IL-1、IL-6、TNF-α及IFN-γ的分泌达到抑制炎症发生,从而保护结肠黏膜。
Objective To detect mechanism of action mode of Jiuxieling Granules in spleen and kidney yang deficiency ulcerative colitis. Methods The perfusion of Rhei Radix et Rhizoma Decoction plus intramuscular injection of hydrocortisone and combined with TNBS and ethanol enema were employed to establish UC animal model. Ninety rats were randomly divided into blank group, model group, SASP group and Jiuxieling Granules 7 days, 14 days and 21 days groups. All treatment groups received relevant medicine intervention. The levels of IL-1, IL-6, TNF-α, and IFN-γin serum and colon tissue were detected by ELISA. Results Compared with the blank group, the levels of IL-1, IL-6, TNF-α, and IFN-γ in serum and colon tissues of rats in model group increased （P〈0.05）;compared with the model group, the levels of IL-1, IL-6, TNF-α, and IFN-γin serum and colon tissues of rats in treatment groups were reduced greatly （P〈0.05）, among which Jiuxieling Granules 21 days group showed the most obvious effects （P〈0.05）. Conclusion Jiuxieling Granules can regulate the normal secretion of the levels of IL-1, IL-6, TNF-α, and IFN-γin serum and colon tissue of model rats, and inhibit inflammation and protect colonic mucosa.