文献类型：期刊文献

中文题名：HPLC同时测定川贝止咳露中的5种成分及指纹图谱研究

英文题名：Simultaneous determination of five components in Chuanbei Zhike syrup by HPLC and study of fingerprints

作者：王苗[1];余磊[2];周德来[1];李运[3];程显隆[4];杨扶德[1]

第一作者：王苗

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]甘肃中医药大学图书馆,甘肃兰州730000;[3]兰州市食品药品检验检测研究院,甘肃兰州730050;[4]中国食品药品检定研究院,北京100050

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2023

卷号：38

期号：4

起止页码：419

中文期刊名：华西药学杂志

外文期刊名：West China Journal of Pharmaceutical Sciences

收录：CSTPCD;;CSCD:【CSCD_E2023_2024】；

基金：国家药品标准物质协作研究与标定项目(2021-W0204001);兰州市2019年第一批科技计划项目(2019-1-60)。

语种：中文

中文关键词：川贝止咳露;高效液相色谱法;指纹图谱;含量测定;新绿原酸;桑皮苷A;绿原酸;隐绿原酸;白花前胡甲素

外文关键词：Chuanbei Zhike syrup;HPLC;Fingerprints;Content determination;Neochlorogenic acid;Mulberroside A;Chlorogenic acid;Cryptochlorogenic acid;Praeruptorin A

摘要：目的建立川贝止咳露的HPLC指纹图谱及5种成分的含量测定方法。方法采用CAPCELL PAK-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%甲酸溶液,流速1.0 mL·min^(-1),柱温30℃,检测波长323 nm。结果41批样品有26个共有峰,相似度为0.22~0.99;5种成分在各自浓度范围内的线性关系良好(r≥0.999),平均加样回收率为96.23%~100.21%,RSD均<3%。其中,Y2厂家样品中未检测到桑皮苷A,其余成分均可检测到。结论所用方法简单可行、快速准确、重复性好,可为川贝止咳露的质量评价提供参考。
OBJECTIVE To establish an HPLC fingerprint and to detect five components in Chuanbei Zhike syrup.METHODS CAPCELL PAK-C_(18) column(250 mm×4.6 mm,5μm)was used.The mobile phase consisted of acetonitrile-0.1%formic acid aqueous solution in gradient manner with a flow rate of 1.0 mL·min^(-1) and a column temperature of 30℃,and the detection wavelength was 323 nm.RESULTS A total of 26 peaks were shared among the 41 batches of samples,with the similarities ranging from 0.22 to 0.99.The results showed that the five components had good linear relationship in their respective ranges(r≥0.999),the average recovery ranged from 96.23%to 100.21%and the RSDs was less than 3%.Mulberroside A was not detected in the samples of manufacturer Y2,and the remaining components were detected.CONCLUSION The method is simple and feasible,rapid and accurate,with good reproducibility,and can provide a reference for the quality evaluation of Chuanbei Zhike syrup.