文献类型：期刊文献

英文题名：Alterations of the rhizosphere soil microbial community composition and metabolite profiles of Angelica sinensis seedlings by co-application of Nitrogen fixing bacteria and amino acids

作者：Peng, Tong[1];Meng, Lingyu[1];Wang, Yinquan[1,2];Jin, Ling[1,2];Jin, Hui[3];Yang, Tao[4];Yao, Yangyang[1]

第一作者：彭涛

通信作者：Wang, YQ[1];Jin, L[1];Wang, YQ[2];Jin, L[2]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou, Peoples R China;[3]Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou, Peoples R China;[4]Gansu Acad Sci, Key Lab Microbial Resources Exploitat & Applicat, Inst Biol, Lanzhou, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]corresponding author), Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2023

卷号：493

期号：1-2

起止页码：535

外文期刊名：PLANT AND SOIL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001063895600001)】；

基金：This work was supported by the Strategic Research and Consulting Project of the Chinese Academy of Engineering (GS2021ZDA06), Double First-Class Major Scientific Research Project of Gansu Provincial Department of Education (GSSYLXM-05), Open fund project of the collaborative innovation center jointly built by the provinces and ministries of Chinese and Tibetan medicine in Northwest China (Xbzzy-2022-01), GKJ [2022] No.12 Gansu Excellent Doctoral Program (22JR5RA580).

语种：英文

外文关键词：Angelica sinensis; Nitrogen-fixing bacteria; Amino acids; C and N metabolism; Rhizosphere soil microbial community

摘要：Aims Our objective was to examine short-term responses of A.sinensis agronomic, soil productivity to agricultural management (i.e. amino acid (AAs) and nitrogen-fixing bacteria (NFB) as alone or co-applicationed exogenous nitrogen (N) after cover planting).Methods A field experiment was established with four fertilization treatments, including NFB (Y1), NFB and AAs (Y2), AAs (Y3), and sterile water (CK). The plant yield, biomass, N and C content, photosynthesis and the soil chemical properties, microbial community and metabolite spectrum were evaluated.Results Y2 improved the crop biomass by 32-66% than CK, Y1 and Y3. It also decreased soil C/N by 24.29% than CK. 16S rRNA and ITS genes revealed that Y2 markedly enhanced the growth of copiotrophic taxa (Alloprevotella, Bactriodies and Alistipes) while inhibiting the growth of oligotrophic taxa (Sphingomonas and Ellin6055), among the dominant bacteria. Y2 reduced the growth of pathogenic taxa (Thanatephorus and Fusarium) that are encouraged by AAs. Further, AAs and NFB addition directly affected the diversity of bacteria and fungi and indirectly affected fungal diversity by altering soil total organic carbon (TOC), total nitrogen (TN), C/N. Additionally, soil metabolomics analysis revealed that Y3 elevated the concentration of chemical compounds that impede microbial growth, while decreasing fructose, N-Acetylglutamic acid, and arginine involved in C and N metabolism. Y2 augmented the content of disease-resistant compounds and lysine in N metabolism.Conclusion Inoculating AAs with NFB (Bacillus amyloliquefaciae and Pseudomonas fluorescence) is a strategy with immense benefits as a biofertilizer for sustainable A.sinensis production, which also renders soil ecological services.