文献类型：期刊文献

中文题名：菊花-茶防治高血压合并高脂血症最佳配比筛选及生物信息学作用机制研究

英文题名：Screening of the optimal ratio of chrysanthemum-tea for prevention and treatment of hypertension complicated with hyperlipidemia and study on its bioinformatic mechanism

作者：李彦荣[1];陈静[2];陈振东[3];颜鲁林[4];郭文龙[4];蒋宜伟[5]

第一作者：李彦荣

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730101;[2]甘肃中医药大学人文与外国语学院,甘肃兰州730101;[3]甘肃中医药大学中西医结合学院,甘肃兰州730101;[4]甘肃中医药大学公共卫生学院,甘肃兰州730101;[5]甘肃中医药大学中医临床学院,甘肃兰州730101

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2026

卷号：43

期号：1

起止页码：27

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：国家自然科学基金地区项目(82360905);甘肃省自然基金项目(21JR1RA268);定西市科技计划项目(DX2021AZ04);甘肃省教育厅高校教师创新基金项目(2023B-116)。

语种：中文

中文关键词：高血压;高脂血症;菊花-茶;网络药理学;分子对接;高效液相色谱;主成分分析;机制研究

外文关键词：hypertension;hyperlipidemia;chrysanthemum-tea;network pharmacology;molecular docking;highperformance liquid chromatography;principal component analysis;mechanism research

摘要：目的研究菊花-茶防治高血压合并高脂血症的药效物质、溶出量与配比的关系及作用机制。方法采用高效液相色谱(HPLC)法测定菊花-茶不同配比中主要药效物质含量,采用主成分分析法对不同配比中主要药效物质含量进行分析。通过中药系统药理学数据库与分析平台(TCMSP)检索菊花-茶的活性成分及靶点,基于GeneCards、UniProt数据库获取高血压合并高脂血症的靶点,并确定菊花-茶干预高血压合并高脂血症的共同靶点;利用DAVID数据库进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析,并通过分子对接技术进行验证。结果菊花-茶配比为1∶3时药效物质溶出量最高。菊花-茶中主要活性成分为槲皮素、木犀草素、表没食子儿茶素没食子酸酯、山奈酚等,核心靶点为蛋白激酶B(AKT1)、丝裂原活化蛋白激酶1(MAPK1)、肿瘤蛋白p53(TP53)、v-rel网状内皮增生病毒癌基因同源物A(RELA)等。GO与KEGG富集分析显示,菊花-茶主要活性成分干预高血压合并高脂血症与晚期糖基化终产物-晚期糖基化终末产物受体(AGE-RAGE)、白细胞介素(IL)-17、肿瘤坏死因子(TNF)等信号通路相关。分子对接结果显示,主要活性成分与核心靶点具有较强的结合能力。结论菊花-茶防治高血压合并高脂血症的最佳配比为1∶3,菊花-茶通过多成分、多靶点、多途径防治高血压合并高脂血症。
Objective To investigate the relationship between the ratio of chrysanthemum-tea and its pharmacodynamic substance dissolution,as well as the mechanism of action in preventing and treating hypertension complicated with hyperlipidemia.Methods The content of main pharmacodynamic substances in different ratios of chrysanthemum-tea was determined by high-performance liquid chromatography(HPLC).Principal component analysis(PCA)was used to analyze the content of these substances.Active ingredients and targets of chrysanthemum-tea were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).Targets related to hypertension complicated with hyperlipidemia were obtained from GeneCards and UniProt databases,and common targets for chrysanthemum-tea intervention were identified.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed using the DAVID database,and results were validated by molecular docking technology.Results The highest dissolution of pharmacodynamic substances was achieved at a chrysanthemum-tea ratio of 1:3.The main active components included quercetin,luteolin,epigallocatechin gallate,and kaempferol.Core targets included protein kinase B(AKT1),mitogen-activated protein kinase 1(MAPK1),tumor protein p53(TP53),and v-rel reticuloendotheliosis viral oncogene homolog A(RELA).GO and KEGG enrichment analyses indicated that the intervention primarily involved signaling pathways such as AGE-RAGE,interleukin(IL)-17,and tumor necrosis factor(TNF).Molecular docking results demonstrated strong binding affinity between the main active components and core targets.Conclusion The optimal ratio of chrysanthemum-tea for preventing and treating hypertension complicated with hyperlipidemia is 1:3.The mechanism involves multiple components,targets,and pathways.