文献类型：期刊文献

中文题名：Actions of genistein on contractile response of smooth muscle isolated from guinea pig gallbladder

英文题名：Actions of genistein on contractile response of smooth muscle isolated from guinea pig gallbladder

作者：Luo, Ya-Li[1];Wang, Ya-Li[1];Li, Neng-Lian[1];Zheng, Tian-Zhen[2];Zhang, Li[1];She, Ya-Li[1];Hu, Shu-Ming[1]

第一作者：骆亚莉

通信作者：Luo, YL[1]

机构：[1]Gansu Coll Tradit Chinese Med, Dept Pathol, Dept Basic Med, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Dept Physiol, Coll Basic Med, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Coll Tradit Chinese Med, Dept Pathol, Dept Basic Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2009

卷号：8

期号：6

起止页码：614

中文期刊名：国际肝胆胰疾病杂志：英文版

外文期刊名：HEPATOBILIARY & PANCREATIC DISEASES INTERNATIONAL

收录：;Scopus(收录号：2-s2.0-73849139436);WOS:【SCI-EXPANDED(收录号:WOS:000272607300009)】；PubMed;

语种：英文

中文关键词：genistein;isolated;gallbladder;smooth;muscle;calcium;channel;H2;receptor;guinea;pigs

外文关键词：genistein; isolated gallbladder smooth muscle; calcium channel; H-2 receptor; guinea pigs

摘要：BACKGROUND:Defective contractile motility of the gallbladder is an important factor for gallstone formation.Estrogen might increase the risk of gallstones and cholecystitis,and estradiol inhibits the contractile activity of isolated strips of guinea pig gallbladder.The potential risks associated with hormone replacement therapy(HRT) include symptomatic gallstones.Phytoestrogen have been used to treat menopause syndromes by replacing traditional estrogen.This experiment aimed to determine the effects of the phytoestrogen genistein on the contractile response of smooth muscle strips isolated from guinea pig gallbladder and its possible mechanism of action.METHODS:Guinea pigs were sacrificed to remove the whole gallbladder.Two or three smooth muscle strips were cut longitudinally.Each strip was suspended in a tissue chamber containing Krebs solution.After 2 hours of equilibration,contractile response indexes were recorded.Different concentrations of genistein were added to the chamber and the contractile responses were measured.Each antagonist was added 2 minutes before genistein to study possible mechanisms.The effect of genistein on calcium-dependent contraction curves and biphasic contraction in calcium-free Krebs solution were measured.RESULTS:Genistein decreased the resting tension dose-dependently,and reduced the mean contractile amplitude and frequency in gallbladder strips.Ranitidine partly inhibited the effect of genistein,but methylene blue,Nω-nitro-L-arginine,and propranolol hydrochloride did not influence this action.Genistein had no significant effects on calcium-dependent contraction.Genistein reduced the first contraction induced by acetylcholine chloride,but did not affect the second contraction caused by CaCl2.CONCLUSIONS:Genistein relaxed smooth muscle isolated from the gallbladder of guinea pigs and this might contribute to the formation of gallstones.The inhibitory action might be related to H2 receptors and the release of intracellular Ca 2+ from sarcoplasmic reticulum.Replacing traditional estrogen with phytoestrogen to treat menopause syndromes may increase the risk of gallstone formation.
BACKGROUND: Defective contractile motility of the gallbladder is an important factor for gallstone formation. Estrogen might increase the risk of gallstones and cholecystitis, and estradiol inhibits the contractile activity of isolated strips of guinea pig gallbladder. The potential risks associated with hormone replacement therapy (HRT) include symptomatic gallstones. Phytoestrogen have been used to treat menopause syndromes by replacing traditional estrogen. This experiment aimed to determine the effects of the phytoestrogen genistein on the contractile response of smooth muscle strips isolated from guinea pig gallbladder and its possible mechanism of action. METHODS: Guinea pigs were sacrificed to remove the whole gallbladder. Two or three smooth muscle strips were cut longitudinally. Each strip was suspended in a tissue chamber containing Krebs solution. After 2 hours of equilibration, contractile response indexes were recorded. Different concentrations of genistein were added to the chamber and the contractile responses were measured. Each antagonist was added 2 minutes before genistein to study possible mechanisms. The effect of genistein on calcium-dependent contraction curves and biphasic contraction in calcium-free Krebs solution were measured. RESULTS: Genistein decreased the resting tension dose-dependently, and reduced the mean contractile amplitude and frequency in gallbladder strips. Ranitidine partly inhibited the effect of genistein, but methylene blue, N-omega-nitro-L-arginine, and propranolol hydrochloride did not influence this action. Genistein had no significant effects on calcium-dependent contraction. Genistein reduced the first contraction induced by acetylcholine chloride, but did not affect the second contraction caused by CaCl2. CONCLUSIONS: Genistein relaxed smooth muscle isolated from the gallbladder of guinea pigs and this might contribute to the formation of gallstones. The inhibitory action might be related to H-2 receptors and the release of intracellular Ca2+ from sarcoplasmic reticulum. Replacing traditional estrogen with phytoestrogen to treat menopause syndromes may increase the risk of gallstone formation.