文献类型：期刊文献

中文题名：菖蒲郁金汤对抽动-秽语综合征模型大鼠纹状体神经递质含量及其病理损伤的动态影响

英文题名：Dynamic effects of Changpu Yujin Tang(菖蒲郁金汤)on neurotransmitter content of striatum and pathological damage of Tourette syndrome in model rats

作者：冯鹏[1,2];史正刚[1];尚菁[1];罗文珍[1];孙治前[1];路曼琪[1];李玉霞[3];田文霞[3];吴丽萍[3];陈静[3]

第一作者：冯鹏

机构：[1]甘肃中医药大学中医临床学院,甘肃兰州730101;[2]河西学院医学院,甘肃张掖734000;[3]甘肃中医药大学附属医院儿科,甘肃兰州730020

第一机构：甘肃中医药大学中医临床学院

年份：2022

卷号：18

期号：5

起止页码：1

中文期刊名：中医儿科杂志

外文期刊名：Journal of Pediatrics of Traditional Chinese Medicine

基金：国家自然科学基金地区资助项目(81960886);甘肃省高等学校创新基金项目(2020A-081);甘肃省重点研发计划项目(20YF3FA041);甘肃省优秀研究生“创新之星”项目(2021CXZX-764)。

语种：中文

中文关键词：抽动-秽语综合征;菖蒲郁金汤;多巴胺;高香草酸;大鼠;实验研究

外文关键词：Tourette syndrome(TS);Changpu Yujin Tang(菖蒲郁金汤,CPYJT);dopamine(DA);homovanillic acid(HVA);rats;experimental study;

摘要：目的探讨菖蒲郁金汤治疗儿童抽动-秽语综合征(Tourette syndrome,TS)的可能作用机制。方法120只SD幼龄大鼠随机分为空白组(30只)和造模组(90只),造模组采用亚氨基二丙腈(IDPN)进行造模,造模成功后,再次随机分为模型组、硫必利组、菖蒲郁金汤组,每组30只。硫必利组给予硫必利药液(3.194 mg/mL)、菖蒲郁金汤组予以菖蒲郁金汤药液(5.152 g/mL)、空白组和模型组予以等体积生理盐水,以上各组均按15 mL/(kg·d)灌胃,每日1次,持续28 d。记录各组大鼠在造模期间及治疗期间的一般情况和体质量;在造模后及干预第7,14,21,28 d采用刻板及运动行为评分法观察大鼠行为学的改善情况;观察结束后麻醉大鼠,取大鼠纹状体,运用酶联免疫吸附测定(ELISA)法检测纹状体多巴胺(DA)、高香草酸(HVA)含量,运用HE染色法观察纹状体组织的形态结构。结果造模结束后造模组大鼠行为学评分均≥2,说明造模成功。与空白组比较,模型组、硫必利组、菖蒲郁金汤组大鼠纹状体中DA、HVA含量显著下降,差异有统计学意义(P<0.05),组间比较差异无统计学意义(P>0.05),说明3组大鼠纹状体病理损伤明显。在干预期间硫必利组和菖蒲郁金汤组行为学评分随着干预时间的延长逐渐下降,DA、HVA含量逐渐升高。干预结束后硫必利组和菖蒲郁金汤组行为学评分与模型组比较显著下降(P<0.05),菖蒲郁金汤组行为学评分均低于硫必利组(P<0.05),2组DA、HVA较空白组低(P<0.05),较模型组高(P<0.05),菖蒲郁金汤组DA、HVA含量低于硫必利组,但差异无统计学意义(P>0.05)。菖蒲郁金汤组纹状体病理学损伤改善明显,模型组和硫必利组无明显改善。结论菖蒲郁金汤能明显改善TS模型幼龄大鼠的抽动症状,其可能的机制是调控DA、HVA的含量,并有修复神经元的作用。
Objective To explore the possible mechanism of Changpu Yujin Tang(菖蒲郁金汤,CPYJT)in the treatment of children Tourette syndrome(TS).Methods Totally 120 SD rats were randomly divided into blank group(30 ones)and establishing model group(90 ones).The establishing model used 3,3′-iminodiproprionitrile(IDPN)to establish models.After the model was successfully established,they were subdivided into model group,tiapride hydrochloride group,CPYJT group,with 30 rats in each group.Tiapride hydrochloride group was given tiapride medicated liquid(3.194 mg/mL),CPYJT group was given CPYJT medicated liquid(5.152 g/mL),blank group and model group were given equal volume of normal saline,the above groups were given 15 mL/(kg·d)by gavage once a day for 28 days.The general condition and body mass of the rats in each group during the establishing model period and the treatment period were recorded.After establishing modeling and treatment for 7 days,14 days,21 days and 28 days,the behavioral improvement of the rats was observed by the stereotypic and motor behavior scale method.At the end of observation,the rat striatum was taken after they were anesthetized,and the content of dopamine(DA)and homovanillic acid(HVA)in the striatum was detected by ELISA method,and the morphological structure of striatum tissue was observed by HE staining method.Results After the model was established,the behavioral scores of rats in the establishing model group were all≥2,indicating that the establishing model was successful.The content of DA and HVA in rat striatum of model group,tiapride hydrochloride group and CPYJT group decreased significantly as compared with blank group,and the difference was statistically significant(P<0.05).There was no significant difference among groups(P>0.05),indicating that the pathological damage was significant in rat striatum of the 3 groups.During the intervention period,the behavioral scores of tiapride hydrochloride group and CPYJT group gradually decreased with the extension of the intervention time,and the content of DA and HVA therein gradually increased.After the intervention,the behavioral scores of tiapride hydrochloride group and CPYJT decreased significantly as compared with model group(P<0.05),the behavioral scores of the latter were lower than that of the former(P<0.05).Content of DA and HVA of the 2 groups were lower as compared with blank group(P<0.05),whereas they were higher as compared with model group(P<0.05),the DA and HVA content of CPYJT group were lower than those of tiapride hydrochloride group,but the difference was not statistically significant(P>0.05).The pathological damage of the striatum in CPYJT group was significantly improved,but there was no significant improvement in model group and tiapride hydrochloride group.Conclusion CPYJT can markedly improve the tic symptoms of TS model rats,whose possible mechanism is to regulate the content of DA and HVA,with the effect of repairing neurons.