文献类型：期刊文献

英文题名：In vitro bioactive metabolite production and plant regeneration of medicinal plant Angelica sinensis

作者：Huang, Tianmiao[1]; Liu, Di[1]; Cui, Xiuwen[1]; Li, Meiling[1]; Jin, Ling[2]; Paré, Paul W.[3]; Li, Mengfei[1]; Wei, Jianhe[4]

第一作者：Huang, Tianmiao

机构：[1] State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070, China; [2] College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, 730000, China; [3] Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, 79409, United States; [4] Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China

第一机构：State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070, China

通信机构：[1]State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070, China

年份：2023

卷号：194

外文期刊名：Industrial Crops and Products

收录：EI(收录号：20230413418630);Scopus(收录号：2-s2.0-85146433528)

语种：英文

外文关键词：Biomolecules - Cells - Geographical distribution - Medicine - Plant cell culture - Plants (botany)

摘要：The root of Angelica sinensis (Oliv.) Diels is a traditional Chinese medicine (TCM) widely used for the cardio-cerebrovascular, anti-inflammatory, and anti-cancer agents, etc. Currently, the root yield is mainly limited by narrow geographical distribution, early bolting and flowering (EBF), continuous cropping obstacle, and germplasm degeneration. While two ancillary strategies to produce bioactive metabolites by bioreactor and innovate new germplasms by genome editing have not been conducted. Here is reported embryogenic callus induction, histomorphological structure identification, suspension cell culture, bioactive metabolite determination, shoot bud and root differentiation, and plantlet acclimatization at different treatments such as explants, hormones, and culture conditions. The embryogenic calli can be effectively induced (99.0%) from the root of aseptic seedlings by a combination of 2.0 mg/L IBA and 0.2 mg/L KT, and embryogenic cells including: three-cell, globular-, heart-, torpedo-, and cotyledon-shaped embryos were observed. The maximum growth rate, bioactive metabolites contents (i.e., ferulic acid, soluble sugar, flavonoids, and phenolic), and antioxidant capacities were observed at 9–12 d with inoculum density 40 g/L, 22 ℃ and 16/8 h light/dark. Robust plantlets can be obtained within 180 d after successive shoot bud and root differentiation as well as plantlet acclimation. Based on the strategies for the satisfaction of root demand, an effective and complete scheme is proposed for producing bioactive metabolites and regenerating plant of A. sinensis. ? 2023 Elsevier B.V.