文献类型：期刊文献

英文题名：Separation of astragaloside IV from Astragalus membranaceus based on high-speed countercurrent chromatography in continuous injection mode

作者：Zhao, Yin-Di[1,2];Su, Qian-Xia[1,2];Wang, Ning-Li[2];Pei, Dong[2];Huang, Xin-Yi[1,2,3,4]

第一作者：赵耀东;Zhao, Yin-Di

通信作者：Pei, D[1];Huang, XY[1]

机构：[1]Gansu Univ Tradit Chinese Med, Sch Pharm, Lanzhou, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[3]Yunnan Minzu Univ, State Ethn Affairs Commiss, Key Lab Chem Ethn Med Resources, Kunming, Yunnan, Peoples R China;[4]Yunnan Minzu Univ, Minist Educt, Kunming, Yunnan, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China.

年份：2024

外文期刊名：PHYTOCHEMICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001298538400001)】；

基金：National Natural Science Foundation of China, Grant/Award Number: 22074155; CAS "Young Scholars for Regional Development" program, Grant/Award Number: 2022-045; National Natural Sciences Foundation of China; Gansu Province Intellectual Property Plan Project, Grant/Award Number:23ZSCQG026; Key Research and Development Program of Gansu Province, Grant/Award Number: 22YF7NA027; "Longyuan Youth Talent" Program

语种：英文

外文关键词：astragaloside IV; continuous separation; high-speed countercurrent chromatography; injection mode

摘要：Introduction: Astragaloside IV (AS-IV) is an index for the quality evaluation of the traditional Chinese medicine Astragalus and an important material basis for Astragalus to exert its medicinal effects, and it is difficult to obtain a single AS-IV by ordinary separation methods. Objective: To find a new isolation method that can prepare AS-IV quickly and efficiently. Methodology: AS-IV was isolated from Astragalus membranaceus extract by high-speed countercurrent chromatography using a two-phase solvent system consisting of ethyl acetate/n-butanol/water (4.2:0.8:5, v/v) at a speed of 950 rpm at a flow rate of 2 mL/min using one of the high-speed countercurrent chromatographic sequential injection models developed during the previous study. Results: Compared with the common countercurrent chromatographic separation, this separation method increased the injection volume and yield by 4-fold and 4.47-fold, respectively, with only about 1.2-fold increase in solvent consumption and separation time, and the purity was basically not reduced, and 55.9 mg of AS-IV, with a purity of 96.95%, was finally prepared from 400 mg of the crude extract in 240 min. Conclusion: The continuous injection mode of high-speed countercurrent chromatography was able to successfully prepare a large amount of AS-IV with high purity at one time.