文献类型：期刊文献

英文题名：Protective effect of Cistanchis A on ethanol-induced damage in primary cultured mouse hepatocytes

作者：Luo, Huiying[1,2];Cao, Rongrong[1];Wang, Lijuan[1,2];Zhu, Lijuan[1,2]

第一作者：罗慧英;Luo, Huiying

通信作者：Luo, HY[1]

机构：[1]Gansu Univ Chinese Med, AV Dingxi 30, Lanzhou 730000, Peoples R China;[2]Key Lab Pharmacol & Toxicol Tradit Chinese Med Ga, AV Dingxi 30, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, AV Dingxi 30, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2016

卷号：83

起止页码：1071

外文期刊名：BIOMEDICINE & PHARMACOTHERAPY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000390433400128)】；

语种：英文

外文关键词：Cistanoside A; alcohol damage; primary cultured hepatocytes; apoptosis

摘要：Cistanoside A (C. A) was one of phenylethanol glycosides isolated from Cistanche deserticola, a tonic in traditional Chinese medicine. In our previous research, we demonstrated that Cistanoside A (C. A) possess the protective activities on CCl4 induced hepatotoxicity in mice, such as increasing free radicals clearing activities, alleviating lipid-overoxidation damage, and improving respiratory chain function in mitochondria. Meanwhile, our previous research also demonstrated C. A possess protective activities on alcohol induced hepatotoxicity in mice, shown in ameliorate the hepatic function indices, lightening steatosis and inflammatory infiltration, increasing free radicals clearing activities, alleviating lipid-overoxidation damage, and alleviating energy metabolism in mitochondria. The aim of this research was to evaluate the effects of Cistanoside A (C. A) on ethanol-induced damage in primary cultured mouse hepatocytes, and probe into the mechanism related. Using fluorescent staining, flow cytometer, immunohistochemistry analysis, and Western blotting, we demonstrated that C. A could enhance the survival rate of the primary cultured hepatocytes, alleviate apoptosis and necrosis, the mechanism was involved with enhance the expression of apoptosis inhibition factor bcl-2, and inhibition the expression of immediate early genes c-fos. (C) 2016 Elsevier Masson SAS. All rights reserved.