文献类型：期刊文献

中文题名：低频脉冲电磁场治疗绝经后骨质疏松症的实验研究

英文题名：Experimental study on the treatment of postmenopausal osteoporosis with lowfrequency pulsed electromagnetic fields

作者：安梓栋[1,2];王立强[1,2];武艺[1];庞永杰[1];陈克明[1,2];高玉海[1]

第一作者：安梓栋

机构：[1]中国人民解放军联勤保障部队第九四〇医院基础医学实验室,兰州730050;[2]甘肃中医药大学中医临床学院,兰州730000

第一机构：中国人民解放军联勤保障部队第九四〇医院基础医学实验室,兰州730050

年份：2025

卷号：42

期号：5

起止页码：1054

中文期刊名：生物医学工程学杂志

外文期刊名：Journal of Biomedical Engineering

收录：;EI(收录号：20254319393553);Scopus(收录号：2-s2.0-105020358717);北大核心:【北大核心2023】；

基金：第九四〇医院高层次人才项目(2024-G3-10);兰州市科技计划项目(2023-1-8,2023-ZD-183);甘肃省科技重大专项(24ZDFA008)。

语种：中文

中文关键词：骨质疏松症;低频脉冲电磁场;骨密度;骨形成;骨吸收

外文关键词：Osteoporosis;Low-frequency pulsed electromagnetic field;Bone mineral density;Bone formation;Bone resorption

摘要：本研究旨在探讨50 Hz、0.6 mT低频脉冲电磁场(PEMF)对切除卵巢导致的大鼠绝经后骨质疏松症的疗效。选取3月龄雌性SD大鼠30只,分为假手术组(Sham)、切卵巢模型组(OVX)和低频脉冲电磁场治疗组(PEMF),每组10只。8周后检测各组大鼠全身骨密度,治疗组开始接受每日90 min的PEMF刺激,而OVX组仅模拟放置而不通电。干预6周后,所有大鼠处死后检测离体骨密度、显微CT、生物力学、血清生化指标和骨组织相关蛋白。结果显示,手术8周后OVX组骨密度显著低于Sham组,说明造模成功。治疗6周后,与OVX组相比,PEMF组在全身、股骨及椎体骨密度方面均显著升高,Micro-CT分析结果显示骨微结构得到改善,股骨最大载荷和弯曲强度显著增加,血清骨形成标志物水平提高,且成骨相关蛋白表达量增加。研究结果表明50 Hz、0.6 mT PEMF每天90 min处理可有效提升OVX大鼠骨密度、增强骨骼生物力学性能、优化骨微结构,并促进骨形成以及抑制骨吸收,对绝经后骨质疏松具有明显的治疗效果。
This study aims to investigate the therapeutic efficacy of 50 Hz-0.6 mT low-frequency pulsed electromagnetic field(PEMF)on postmenopausal osteoporosis in ovariectomized rats.Thirty 3-month-old female SD rats were selected and divided into a sham operation group(Sham),an ovariectomized model group(OVX),and a lowfrequency pulsed electromagnetic field(PEMF)treatment group,with 10 rats in each group.After 8 weeks,the wholebody bone mineral density(BMD)of each group of rats was measured.The treatment group began to receive PEMF stimulation for 90 minutes daily,while the OVX group only received a simulated placement without electricity.After 6 weeks of intervention,all rats were sacrificed and tested for in vitro BMD,micro-CT,biomechanics,serum biochemical indicators,and bone tissue-related proteins.The results showed that the BMD of the OVX group was significantly lower than that of the Sham group 8 weeks after surgery,indicating successful modeling.After 6 weeks of treatment,compared with the OVX group,the PEMF group exhibited significantly increased BMD in the whole body,femur,and vertebral bodies.Micro-CT analysis results showed improved bone microstructure,significantly increased maximum load and bending strength of the femur,elevated levels of serum bone formation markers,and increased expression of osteogenicrelated proteins.In conclusion,this study demonstrates that daily 90-minute exposure to 50 Hz-0.6 mT PEMF effectively enhances BMD,improves bone biomechanical properties,optimizes bone microstructure,stimulates bone formation,and inhibits bone resorption in ovariectomized rats,highlighting its therapeutic potential for postmenopausal osteoporosis.