文献类型：期刊文献

英文题名：Ampelopsin-sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro

作者：Zhu, Lijuan[1];Zhang, Baolai[1];Luo, Jianyun[3];Dong, Shuhong[1];Zang, Kaihong[2];Wu, Yongjie[1]

第一作者：Zhu, Lijuan

通信作者：Wu, YJ[1]

机构：[1]Lanzhou Univ, Sch Basic Med Sci, Dept Pharmacol, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Dept Pharmacol, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China;[3]Xian Municipal Hlth Commiss, Dept Drug Policy & Essential Med, Xian 710000, Shaanxi, Peoples R China

第一机构：Lanzhou Univ, Sch Basic Med Sci, Dept Pharmacol, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Dept Pharmacol, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2019

卷号：18

期号：1

起止页码：189

外文期刊名：ONCOLOGY LETTERS

收录：;Scopus(收录号：2-s2.0-85068738832);WOS:【SCI-EXPANDED(收录号:WOS:000474896900024)】；

语种：英文

外文关键词：ampelopsin sodium; anti-tumor; apoptosis; human lung adenocarcinoma cell lines; tubulin polymerization

摘要：Previous studies have demonstrated that ampelopsin (AMP), a type of flavonoid isolated from the stems and leaves of Ampelopsis grossedentata, exhibits anti-cancer activity in various types of cancer. Conversion of AMP into its sodium salt (AMP-Na) conferred enhanced solubility and stability to it. The present study aimed to evaluate the anti-cancer activity of AMP-Na in human lung adenocarcinoma cell lines and to investigate its mechanisms of action. Cell proliferation and viability were assessed by MTT and colony formation assays, and cell migration was determined using a scratch wound healing assay. The cell cycle distribution, apoptosis rate and tubulin immunofluorescence intensity were analyzed using flow cytometry, the cell ultra-microstructure was examined using transmission electron microscopy and the accumulation of tubulin was determined using laser confocal microscopy. The results demonstrated that AMP-Na significantly inhibited the proliferation, clonogenicity and migration of human lung adenocarcinoma cells. Furthermore, AMP-Na induced SPC-A-1 cell apoptosis, and promoted tubulin polymerization. The results suggested that the underlying mechanisms of AMP-Na may involve targeting of microtubules and tubulin polymerization to subsequently disrupt mitosis and induce cell cycle arrest at the S-phase.