文献类型：期刊文献

英文题名：The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b

作者：Ding, Nan[1,2];Hua, Junrui[1,2];He, Jinpeng[1,2];Lu, Dong[1,2];Wei, Wenjun[1,2];Zhang, Yanan[1,2];Zhou, Heng[1,2];Zhang, Liying[3];Liu, Yongqi[3];Zhou, Guangming[4];Wang, Jufang[1,2]

第一作者：Ding, Nan

通信作者：Wang, JF[1]

机构：[1]Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Lanzhou 730000, Peoples R China;[4]Soochow Univ, Med Coll, Suzhou 215123, Peoples R China

第一机构：Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China.

年份：2020

卷号：11

期号：8

起止页码：2222

外文期刊名：JOURNAL OF CANCER

收录：;Scopus(收录号：2-s2.0-85080933964);WOS:【SCI-EXPANDED(收录号:WOS:000512898800022)】；

基金：This work was supported by the National Key R&D program of China (grant number: 2017YFC0 108602 and 2017YFC0108605), the National Nature Science Foundation of China (grant number: 11805246 and 31400723); the Hundred-Talent Program of Chinese Academy of Sciences (grant number: Y763050BRO); and the Science and Technology Research Project of Gansu Province (grant number: 145RTSA012).

语种：英文

外文关键词：microRNA; radiation; miR-5094; STAT5b; proliferation

摘要：MicroRNAs (miRNAs) play important roles in the regulation of cellular stress responses. We previously uncovered 10 novel human miRNAs which are induced by X-ray irradiation in HeLa cells using Solexa deep sequencing. The most highly expressed new miRNA, miR-5094, was predicted to target STAT5b. This study wonders whether miR-5094 participates in cellular radiation response via STAT5b. Firstly, direct interaction between miRNA-5094 and the STAT5b 3'-UTR was confirmed by luciferase reporter assay. Then, the radiation responsive expression of miR-5094 and STAT5b were measured in HeLa and Jurkat cells, and the expressions of down-stream genes of STAT5b after ionizing radiation (IR) were detected in HeLa cells. At last, the effects of miR-5094 on survival fraction, cell proliferation, cell cycle arrest and apoptosis induced by IR were investigated in HeLa cells, Jurkat cells and human peripheral blood T cells. It was found that up-regulation of miR-5094 by radiation induction or miRNA mimic transfection suppressed expression of STAT5b, and consequently decreased the transcription of down-stream Igf-1 and Bcl-2. Additionally, over expression of miR-5094 resulted in proliferation suppression and knockdown of miR-5094 by miRNA inhibitor after irradiation partially reversed the proliferation suppression induced by miR-5094 in HeLa cells, Jurkat cells and CD4(+) T cells. Collectively, our findings demonstrate that up-regulation of miR-5094 down-regulated the expression of STAT5b, thereby suppressing cell proliferation after X-ray irradiation.